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Effect of Transmembrane Asymmetric Distribution of Lipids and Peptides on Lipid Bilayers.

08:00 EDT 8th May 2019 | BioPortfolio

Summary of "Effect of Transmembrane Asymmetric Distribution of Lipids and Peptides on Lipid Bilayers."

Transbilayer asymmetry of biomembrane is generated due to the differences in lipid and protein compositions between two leaflets, which plays important roles in physiological functions. However, transbilayer asymmetry can also be originated due to non-equal number of lipids or proteins in each leaflet, which has not been well recognized. Therefore, to shed light on this field, here we generated theoretical models for the effect of transbilayer asymmetry originated from the differences in the number of lipids and peptides in each leaflets on the state of lipid bilayers. The first model described the effect of asymmetric lipid distribution on the state of lipid bilayers. We obtained theoretical equations for the fractional change in area per lipid in both leaflets as a function of the ratio of the number of lipids in each leaflets, which agreed with the molecular dynamics simulation results quantitatively. Results indicated that tensions in both leaflets are opposite in direction and their magnitude is the same. We also performed experiments on the effect of lipid insertion in the outer leaflet on the fractional change in area per lipid. These results agreed quantitatively with the values predicted by the above model. The second model described the effect of asymmetric distribution of peptides on the state of lipid bilayers. We obtained theoretical equations for the area per lipid in both leaflets as a function of the surface concentration of peptides located only in the outer leaflet, which agreed with the results of the antimicrobial peptide magainin 2-induced area change.

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This article was published in the following journal.

Name: The journal of physical chemistry. B
ISSN: 1520-5207
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Medical and Biotech [MESH] Definitions

Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.

Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.

The metabolic process of breaking down LIPIDS to release FREE FATTY ACIDS, the major oxidative fuel for the body. Lipolysis may involve dietary lipids in the DIGESTIVE TRACT, circulating lipids in the BLOOD, and stored lipids in the ADIPOSE TISSUE or the LIVER. A number of enzymes are involved in such lipid hydrolysis, such as LIPASE and LIPOPROTEIN LIPASE from various tissues.

LIPOLYSIS of stored LIPIDS in the ADIPOSE TISSUE to release FREE FATTY ACIDS. Mobilization of stored lipids is under the regulation of lipolytic signals (CATECHOLAMINES) or anti-lipolytic signals (INSULIN) via their actions on the hormone-sensitive LIPASE. This concept does not include lipid transport.

A synthetic phospholipid used in liposomes and lipid bilayers for the study of biological membranes.

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