An Integrated Analysis of Metabolites, Peptides and Inflammation Biomarkers for Assessment of Pre-analytical Variability of Human Plasma.

08:00 EDT 10th May 2019 | BioPortfolio

Summary of "An Integrated Analysis of Metabolites, Peptides and Inflammation Biomarkers for Assessment of Pre-analytical Variability of Human Plasma."

Discrepancies in blood sample collection and processing could have a significant impact on levels of metabolites, peptides and protein biomarkers of inflammation in the blood, thus sample quality control is critical for successful biomarker identification and validation. In this study, we analyzed the effects of several pre-analytical processing conditions, including different storage times and temperatures for blood or plasma samples and different centrifugation forces, on the levels of metabolites, peptides and inflammation biomarkers in human plasma samples using ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. Temperature was found to be the major factor for metabolite variation, and both time and temperature were identified as major factors for peptide variation. For inflammation biomarkers, temperature played different roles depending on the sample type (blood or plasma). Low-temperature affected inflammation biomarkers in blood, while room temperature impacted inflammation biomarkers in plasma.


Journal Details

This article was published in the following journal.

Name: Journal of proteome research
ISSN: 1535-3907


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