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FÖrster resonance energy transfer (FRET)-based biosensors have achieved great success for biological applications. However, what is not extensively appreciated is the growing role as versatile FRET biosensors within a similar biological context. This review provides a brief introduction of recent advances in principle, the designing strategies and kinds of applications of FRET biosensors. For each FRET biosensor, the appropriate background and fabrication is explained before studying their related applications. The prominent roles of nanomaterials are present in the development of more sensitive and specific FRET biosensors. Finally, the challenges and outlooks of FRET biosensors are emphasized.
This article was published in the following journal.
Name: Biosensors & bioelectronics
Two decades have passed since the development of the first calcium indicator based on the green fluorescent protein (GFP) and the principle of Förster resonance energy transfer (FRET). During this pe...
For almost 70 years, Förster resonance energy transfer (FRET) has been investigated, implemented into nowadays experimental nanoscience techniques, and considered in a manifold of optics, photonics a...
Genetically encoded Förster resonance energy transfer (FRET)-based tension sensors measure piconewton-scale forces across individual molecules in living cells or whole organisms. These biosensors sho...
Metallic nanoparticles were shown to affect Förster energy transfer between fluorophore pairs. However, to date, the net plasmonic effect on FRET is still under dispute, with experiments showing effi...
In this paper, we report time resolved fluorescence resonance energy transfer (FRET) using femtosecond ultrafast transient absorption spectroscopy. The lifetimes of FRET are strongly dependent on the ...
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Stray energy transfer during laparoscopic surgery is recognized as a cause of potentially serious complications. This will be the first study to demonstrate clinical evidence of tissue inj...
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The number of copies of mitochondrial genes (mtDNA or "MitoScore") is related to the energy supply of the embryo, which can affect its ability to implant in the maternal uterus. The objec...
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Techniques for determining the proximity of molecules based on ENERGY TRANSFER between bioluminescent chromophores and acceptor fluorophores that have overlapping emission and absorption spectra.
Electric power supply devices which convert biological energy, such as chemical energy of metabolism or mechanical energy of periodic movements, into electrical energy.
A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING.
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...