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The fruit of Nitraria sibirica Pall. (N. sibirica) is a common folk medicine in the Northwest China and contains plentiful antioxidant compositions. In the study, a rapid flash extraction method (extracting for 3.3 min with 53% ethanol at the solvent-sample ratio of 34 mL/g) for the antioxidant compositions in N. sibirica fruit was optimized by response surface methodology. Twenty-seven compounds of the extract were recognized by ultra-high performance liquid chromatography combined with ion trap mass spectrometry (UPLC-IT/MS), including twelve flavonoids, twelve alkaloids and three phenolic acids. Four compounds among them were first reported in Nitraria genus. Moreover, eight main antioxidant compounds in eight batches of N. sibirica fruit collected from different regions were quantified by a rapid and reliable method of ultra-high performance liquid chromatography combined with triple quadrupole tandem mass spectrometry (UPLC-QQQ/MS). Chemometric study further revealed that the contents of two compounds, tryptophan and alcesefoliside, were highly correlated with the antioxidant activity, which provided a reference for the quality evaluation of N. sibirica fruit.
This article was published in the following journal.
Name: Journal of pharmaceutical and biomedical analysis
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An extraction method that separates analytes using a solid phase and a liquid phase. It is used for preparative sample cleanup before analysis by CHROMATOGRAPHY and other analytical methods.
Use of various chemical separation and extraction methods, such as SOLID PHASE EXTRACTION; CHROMATOGRAPHY; and SUPERCRITICAL FLUID EXTRACTION; to prepare samples for analytical measurement of components.
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Nucleotide sequences that are found in the PROMOTER REGIONS of the genes of stress-responsive and cytoprotective proteins, such as those encoding antioxidant and PHASE II DETOXIFICATION enzymes. NF-E2-RELATED FACTOR 2 containing transcription factors bind to these elements during induction of these genes.
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