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Chitinases belong to the group of Pathogenesis-related (PR) proteins that provides protection against the fungal pathogens. In this study, genome-wide identification and characterization of chitinase genes was performed in two important oilseed crops B. juncea and C. sativa belonging to family Brassicaceae. We identified 47 and 79 chitinase genes in B. juncea and C. sativa genome, respectively. Phylogenetic analysis of chitinases in the both species revealed four distinct sub-groups, representing different classes of chitinases (I-V). Microscopic and biochemical parameters highlights the role of ROS scavenging enzymes in disease resistance in B. juncea and C. sativa, Furthermore, qRT-PCR analysis showed that expression of chitinases in both B. juncea and C. sativa was significantly induced by A. brassicae infection. However, the fold change in chitinase expression was considerably higher in C. sativa compared to B. juncea, which further proves the increased resistance of C. sativa to A. brassicae.
This article was published in the following journal.
Dof proteins comprise a plant-specific transcription factor family involved in regulation of many plant processes. Despite their diverse roles, little information is available in cassava (Manihot escu...
Heat shock proteins 70 (Hsp70) constitute a highly conserved protein family of cellular chaperones widely distributed in plants, where they play a fundamental role in response to biotic and abiotic st...
Sucrose plays pivotal role in energy metabolism and regulating gene expression of several physiological processes in higher plants. Here, fourteen sucrose synthase (SUS) genes have been identified in ...
Cellular signaling initiated by various secreted, cysteine-rich Wnt proteins plays essential roles in regulating animal development and cell stemness. By virtue of its functional diversity and importa...
Lipoxygenases (LOXs) (EC 18.104.22.168) catalyze the oxygenation of fatty acids and produce oxylipins including the plant hormone jasmonate (jasmonic acid/methyl jasmonate; MeJA). Little is known about t...
The purpose of this study is to find out the roles of two specific gene families (the chitinase gene family and the TGFB family). We hypothesize that chitinases and TGFb pathway genes will...
Brassica vegetables are an important part of the investigators normal diet and are associated with a reduced risk of many chronic diseases. The protective effect may be as a result of the ...
To reveal the genetic determinants of the treatment outcome of escitalopram in depressed patients (by using candidate gene approach and whole genome scanning).
Some of the liver transplantation recipients experience postoperative acute kidney injury due to various causes including genetic factors. Prevention of postoperative acute kidney injury i...
Despite significant progress made in identification on numerous genes and gene pathways critical for craniofacial development, several approaches, ie mutation screening of specific candida...
A lectin that binds CHITIN, but lacks chitinase activity. It may be involved in tissue remodeling and cellular responses to the environment, including the response of type 2 HELPER T-CELLS to INFLAMMATION and sensitization to ALLERGENS. Mutations in the CHI3L1 gene are associated with ASTHMA.
A plant genus of the family Cruciferae. It contains many species and cultivars used as food including cabbage, cauliflower, broccoli, Brussel sprouts, kale, collard greens, MUSTARD PLANT; (B. alba, B. junica, and B. nigra), turnips (BRASSICA NAPUS) and rapeseed (BRASSICA RAPA).
An analysis comparing the allele frequencies of all available (or a whole GENOME representative set of) polymorphic markers in unrelated patients with a specific symptom or disease condition, and those of healthy controls to identify markers associated with a specific disease or condition.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the longer version of the genome and have no separate cell lysis gene.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
A genetically modified organism (GMO) is an organism whose genetic material has been altered using genetic engineering techniques. Organisms that have been genetically modified include micro-organisms such as bacteria and yeast, insects, plants, fish, an...
Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...