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A Competitive Pull-down Assay Using G-quadruplex DNA Linked Magnetic Nanoparticles to Determine Specificity of G-quadruplex Ligands.

08:00 EDT 16th May 2019 | BioPortfolio

Summary of "A Competitive Pull-down Assay Using G-quadruplex DNA Linked Magnetic Nanoparticles to Determine Specificity of G-quadruplex Ligands."

Herein we develop a new competitive screening method in which G-quadruplex DNA linked magnetic nanoparticles pull down selective ligands for a particular quadruplex topology from a series of small molecules. The screening strategy is first optimized with known G-quadruplex ligands and then used with a series of newly synthesized G-quadruplex interactive bis-triazolyl ligands by Cu(I) catalyzed azide-alkyne cycloaddition. The assay enables the identification of c-MYC and BCL2 G-quadruplex selective bis-triazole ligands that specifically target promoter G-quadruplexes in cancer cells.

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Name: Analytical chemistry
ISSN: 1520-6882
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