Colloidal organosilica spheres for three-dimensional confocal microscopy.

08:00 EDT 16th May 2019 | BioPortfolio

Summary of "Colloidal organosilica spheres for three-dimensional confocal microscopy."

We describe the synthesis and application of 3-(trimethoxysilyl)propyl methacrylate (TPM) particles as a colloidal model system for three-dimensional (3D) confocal scanning laser microscopy. The effect of the initial TPM concentration on the growth and polydispersity of the particles and a recently developed solvent transfer method to disperse particles in a refractive index and density matching solvent mixture are reviewed and discussed. To fully characterise the system as a colloidal model, we measure the pair potential between the TPM particles directly using optical tweezers. Finally, we use 3D confocal microscopy to image a sedimentation-diffusion equilibrium of TPM particles to characterise the phase behaviour and particle dynamics through successful detection and tracking of all particles in the field of view.


Journal Details

This article was published in the following journal.

Name: Langmuir : the ACS journal of surfaces and colloids
ISSN: 1520-5827


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