Advertisement

Topics

Colloidal organosilica spheres for three-dimensional confocal microscopy.

08:00 EDT 16th May 2019 | BioPortfolio

Summary of "Colloidal organosilica spheres for three-dimensional confocal microscopy."

We describe the synthesis and application of 3-(trimethoxysilyl)propyl methacrylate (TPM) particles as a colloidal model system for three-dimensional (3D) confocal scanning laser microscopy. The effect of the initial TPM concentration on the growth and polydispersity of the particles and a recently developed solvent transfer method to disperse particles in a refractive index and density matching solvent mixture are reviewed and discussed. To fully characterise the system as a colloidal model, we measure the pair potential between the TPM particles directly using optical tweezers. Finally, we use 3D confocal microscopy to image a sedimentation-diffusion equilibrium of TPM particles to characterise the phase behaviour and particle dynamics through successful detection and tracking of all particles in the field of view.

Affiliation

Journal Details

This article was published in the following journal.

Name: Langmuir : the ACS journal of surfaces and colloids
ISSN: 1520-5827
Pages:

Links

DeepDyve research library

PubMed Articles [6812 Associated PubMed Articles listed on BioPortfolio]

Confocal Raman microscopy in life sciences.

Microscopy techniques are widely used in life sciences to study cells and tissues. Fluorescence microscopy, for example, is a very common method in many laboratories. While reliable and strong fluores...

Optimizing the synthesis of monodisperse colloidal spheres using holographic particle characterization.

Holographic particle characterization measures the sizes and compositions of individual colloidal particles dispersed in fluid media and rapidly amasses statistics on the distributions of these proper...

Cage dynamics leads to double relaxation of the intermediate scattering function in a binary colloidal system.

A system of binary colloids where one fraction of particles is aggregating by forming irreversible bonds and the other fraction of particles only interacts as hard spheres, is simulated using Brownian...

Facile green synthesis of organosilica nanoparticles by a generic "salt route".

Colloidal silica has wide applications and the global demand of specialty silica is continually increasing. Therefore, it is significant to develop a synthetic method that is simple, versatile, energy...

'Eternal sunshine of the spotless islands': how dermoscopy may influence confocal microscopy when dealing with squamous cells carcinoma simulating basal cell carcinoma.

Basal cell carcinoma (BCC) represents one of the best indications for the use of in-vivo reflectance confocal microscopy (RCM). High levels of diagnostic accuracy is reached with the combined use of d...

Clinical Trials [801 Associated Clinical Trials listed on BioPortfolio]

Pilot Study In Vivo Confocal Microscopy Probe for Cervical Precancer

The goal of this proposal is to determine whether a hand-held confocal microscopy probe may perform better than standard methods in detecting cancer or pre-cancerous abnormalities of the c...

Corneal Confocal Microscopy in Patients With Type 1 Diabetes

Assessing the use of corneal confocal microscopy to evaluate for early neuropathy changes in subjects with Type 1 Diabetes.

Smartphone Confocal Microscopy for Diagnosing Kaposi's Sarcoma

The investigators propose to evaluate a novel diagnostic approach for Kaposi's sarcoma (KS) that may be eventually deployed with portable, point-of-care techniques. This approach features ...

In Vivo Confocal Microscopy for Pigmented Lesion Diagnosis

The purpose of this study is to image pigmented skin lesions suspicious for melanoma with an imaging technology called in vivo reflectance confocal microscopy. This technology uses low int...

In Vivo Imaging of Pigmentary Disorders by Reflectance Confocal Microscopy

This is a pilot study to evaluate the findings of the Reflectance Confocal Microscopy when performed in cutaneous pigmentary disorders, such as vitiligo, melasma, post-inflammatory hyper-p...

Medical and Biotech [MESH] Definitions

Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.

A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.

A type of scanning probe microscopy in which a very sharp conducting needle is swept just a few angstroms above the surface of a sample. The tiny tunneling current that flows between the sample and the needle tip is measured, and from this are produced three-dimensional topographs. Due to the poor electron conductivity of most biological samples, thin metal coatings are deposited on the sample.

A tomographic technique for obtaining 3-dimensional images with transmission electron microscopy.

Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Searches Linking to this Article