Advertisement

Topics

Rapid Detection of Exosomal MicroRNAs Using Virus-Mimicking Fusogenic Vesicles.

08:00 EDT 5th May 2019 | BioPortfolio

Summary of "Rapid Detection of Exosomal MicroRNAs Using Virus-Mimicking Fusogenic Vesicles."

Exosomal microRNAs (miRNAs) are important biomarkers for clinical diagnosis and disease treatment monitoring. However, most approaches for exosomal miRNA detection are time-consuming, laborious, and expensive. Herein, we report a virus-mimicking fusogenic vesicle (Vir-FV) that enables rapid, efficient, and high-throughput detection of exosomal miRNAs within 2 h. Fusogenic proteins on Vir-FVs can specifically target the sialic-acid-containing receptors on exosomes, inducing efficient fusion of Vir-FVs and exosomes. Upon vesicle content mixing, the molecular beacons encapsulated in Vir-FVs specifically hybridize with the target miRNAs in the exosomes, generating fluorescence. Combined with flow cytometry, the Vir-FVs can not only detect exosomal miRNAs but also distinguish tumor exosomes from normal exosomes by sensing the tumor-related miRNAs, paving the way towards the rapid and efficient detection of exosomal miRNAs for diagnosis and prognosis prediction of diseases.

Affiliation

Journal Details

This article was published in the following journal.

Name: Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Pages:

Links

DeepDyve research library

PubMed Articles [13547 Associated PubMed Articles listed on BioPortfolio]

Serum exosomal microRNAs as novel biomarkers for multiple myeloma.

Accumulating studies have focused on circulating microRNAs, which might be potential biomarkers for different malignancies. The aim of this study was to investigate the potential of serum exosomal mic...

Cell-cell fusion induced by reovirus FAST proteins enhances replication and pathogenicity of non-enveloped dsRNA viruses.

Fusogenic reoviruses encode fusion-associated small transmembrane (FAST) protein, which induces cell-cell fusion. FAST protein is the only known fusogenic protein in non-enveloped viruses, and its rol...

Rapid detection of Banna virus by reverse transcription-loop-mediated isothermal amplification (RT-LAMP).

Banna virus (BAV) is classified in the genus Seadornavirus within the Reoviridae family and considered to be an emerging pathogen. We aimed to develop a rapid and simple molecular detection approach f...

Evaluation of a rapid isothermal nucleic acid amplification kit, Alere™ i Influenza A&B, for the detection of avian influenza viruses.

Rapid and accurate diagnosis of influenza virus infection is essential for quick responses for both human and animal health. The Alere™ i Influenza A&B is a novel isothermal nucleic acid amplificati...

Detection of viral components in exosomes derived from NDV-infected DF-1 cells and their promoting ability in virus replication.

Exosomes are micro messengers encapsulating RNA, DNA, and proteins for intercellular communication associated with various physiological and pathological reactions. Several viral infection processes h...

Clinical Trials [5701 Associated Clinical Trials listed on BioPortfolio]

Identification of microRNAs Involved in Cerebral Collateral Regulation

Patients >18 years of age presenting to Ohio State Wexner Medical Center Emergency department with stroke symptoms, within 6 hours of last know well and found to have acute anterior circul...

Non Invasive Detection of Cardiac Allograft Rejection by Circulating microRNAs

The purpose of this study is to evaluate the level of expression of 4 circulating microRNAs in the serum using RT-PCR. A pilote study with cardiac transplant patients has shown that expres...

MicroRNAs as Diagnostic Biomarkers in Hepatocellular Carcinoma Among Somali Patients

Hepatocellular carcinoma (HCC) is the most common primary liver cancer and is the third leading cause of cancer that related death in the worldwide. Although, current most of doctors and l...

Dose-effect Relationship Between microRNAs in Peripheral Blood and Radiation Injury

Rapid and accurate assessment of radiation injury dose is the key to success in early treatment and an urgent issue to be solved in clinical medicine.Researches showed that the expression ...

Circulating microRNAs as Disease Markers in Pediatric Cancers

MicroRNAs are small molecules which have recently been discovered in cells. They are known to be responsible for the normal development of cells and when they are disrupted can contribute...

Medical and Biotech [MESH] Definitions

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

Ascertaining of deception through detection of emotional disturbance as manifested by changes in physiologic processes usually using a polygraph.

Found in large amounts in the plasma and urine of patients with malignant melanoma. It is therefore used in the diagnosis of melanoma and for the detection of postoperative metastases. Cysteinyldopa is believed to be formed by the rapid enzymatic hydrolysis of 5-S-glutathionedopa found in melanin-producing cells.

MicroRNAs found in cell-free BODY FLUIDS such as SERUM; PLASMA; SALIVA; OR URINE.

DNA sequences that form the coding region for a trans-activator protein that specifies rapid growth in human immunodeficiency virus (HIV). vpr is short for viral protein R, where R is undefined.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Searches Linking to this Article