Feasibility of using dried blood spots for HIV viral load testing among HIV-infected individuals in Thailand using QIAGEN QIAsymphony-artus HIV-1 platform.

08:00 EDT 16th May 2019 | BioPortfolio

Summary of "Feasibility of using dried blood spots for HIV viral load testing among HIV-infected individuals in Thailand using QIAGEN QIAsymphony-artus HIV-1 platform."

In settings where plasma preparation and sample centralization are not feasible or inconvenient, dried blood spots (DBS) could be used as alternative specimen to plasma to assess antiretroviral treatment response among HIV-infected individuals. This study was aimed to 1) validate the recent QIAsymphony-artus assay for DBS HIV VL and 2) assess the feasibility of measuring HIV viral load (VL) on DBS using this assay in Thailand. EDTA-blood samples from 99 HIV-infected individuals were used to prepare paired DBS and plasma. Also, DBS samples were shipped to three distant hospitals in northern region. After short-term storage, DBS were returned by regular post to the AMS laboratory and were re-tested for HIV VL using the same platform. HIV VL results were compared using Pearson's correlation and Bland-Altman analysis. DBS HIV VL fairly correlated to plasma HIV VL (R=0.62) with a mean difference of 0.02 log IU/mL (SD=1.06). A high correlation (R=0.79) was observed between HIV VL in DBS before and after shipping (mean difference=0.14 log IU/mL, SD=0.74), indicating a good stability of HIV RNA in DBS. DBS can be used as an alternative specimen for HIV VL monitoring in Thailand. However, measurement of HIV VL with the QIAGEN QIAsymphony-artus assay should be improved, especially the DBS pre-extraction process. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: Journal of medical virology
ISSN: 1096-9071


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