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In settings where plasma preparation and sample centralization are not feasible or inconvenient, dried blood spots (DBS) could be used as alternative specimen to plasma to assess antiretroviral treatment response among HIV-infected individuals. This study was aimed to 1) validate the recent QIAsymphony-artus assay for DBS HIV VL and 2) assess the feasibility of measuring HIV viral load (VL) on DBS using this assay in Thailand. EDTA-blood samples from 99 HIV-infected individuals were used to prepare paired DBS and plasma. Also, DBS samples were shipped to three distant hospitals in northern region. After short-term storage, DBS were returned by regular post to the AMS laboratory and were re-tested for HIV VL using the same platform. HIV VL results were compared using Pearson's correlation and Bland-Altman analysis. DBS HIV VL fairly correlated to plasma HIV VL (R=0.62) with a mean difference of 0.02 log IU/mL (SD=1.06). A high correlation (R=0.79) was observed between HIV VL in DBS before and after shipping (mean difference=0.14 log IU/mL, SD=0.74), indicating a good stability of HIV RNA in DBS. DBS can be used as an alternative specimen for HIV VL monitoring in Thailand. However, measurement of HIV VL with the QIAGEN QIAsymphony-artus assay should be improved, especially the DBS pre-extraction process. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: Journal of medical virology
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The quantity of measurable virus in a body fluid. Change in viral load, measured in plasma, is sometimes used as a SURROGATE MARKER in disease progression.
Techniques for using whole blood samples collected on filter paper for a variety of clinical laboratory tests.
Testing erythrocytes to determine presence or absence of blood-group antigens, testing of serum to determine the presence or absence of antibodies to these antigens, and selecting biocompatible blood by crossmatching samples from the donor against samples from the recipient. Crossmatching is performed prior to transfusion.
The continuous, long-term suppression of VIRAL LOAD, generally to undetectable levels, as the result of treatment with ANTIVIRAL AGENTS.
Method in which repeated blood pressure readings are made while the patient undergoes normal daily activities. It allows quantitative analysis of the high blood pressure load over time, can help distinguish between types of HYPERTENSION, and can assess the effectiveness of antihypertensive therapy.
AIDS and HIV
AIDS; Acquired Immune Deficiency Syndrome. HIV; Human Immunodeficiency Virus HIV infection causes AIDS. HIV infection also causes the production of anti-HIV antibodies, which forms the test for HIV in patients. People who have the HIV antibodies are ...