Central Composite Design for Dispersive Liquid-liquid Microextraction of 25-hydroxy-cholecalciferol in Human Serum.

08:00 EDT 16th May 2019 | BioPortfolio

Summary of "Central Composite Design for Dispersive Liquid-liquid Microextraction of 25-hydroxy-cholecalciferol in Human Serum."

The human body's vitamin D levels are determined by measuring the level of 25-hydroxy-vitamin D3 (25-OH-vitamin D3) in human serum. In this research, a fast, simple, efficient and highly sensitive low-density solvent based on dispersive liquid-liquid microextraction (DLLME) was employed for the successful determination of 25-OH-vitamin D3 from complex human serum matrices. Reversed phase high-performance liquid chromatography was used as a powerful technique. The important parameters in the low-density solvent-DLLME method were optimized using response surface methodology based on central composite design. The calibration curves displayed a high level of linearity (R2 > 0.997) for 25-OH-vitamin D3 in the range of 2-500 ng mL-1. The limit of detection and limit of quantitation were 0.6 ng mL-1 and 1.9 ng mL-1, respectively. The relative standard deviation for the seven analyses was 7.1%. The relative recoveries of vitamin D3 in spiked human serum samples were between 85% and 97%. The amount of 25-OH-vitamin D3 in samples was determined using the proposed method and acceptable results were reported.


Journal Details

This article was published in the following journal.

Name: Journal of chromatographic science
ISSN: 1945-239X


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Medical and Biotech [MESH] Definitions

Miniaturized methods of liquid-liquid extraction.

The removal of a soluble component from a liquid mixture by contact with a second liquid, immiscible with the carrier liquid, in which the component is preferentially soluble. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)

Chromatographic techniques in which the mobile phase is a liquid.

Materials in intermediate state between solid and liquid.

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