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The human body's vitamin D levels are determined by measuring the level of 25-hydroxy-vitamin D3 (25-OH-vitamin D3) in human serum. In this research, a fast, simple, efficient and highly sensitive low-density solvent based on dispersive liquid-liquid microextraction (DLLME) was employed for the successful determination of 25-OH-vitamin D3 from complex human serum matrices. Reversed phase high-performance liquid chromatography was used as a powerful technique. The important parameters in the low-density solvent-DLLME method were optimized using response surface methodology based on central composite design. The calibration curves displayed a high level of linearity (R2 > 0.997) for 25-OH-vitamin D3 in the range of 2-500 ng mL-1. The limit of detection and limit of quantitation were 0.6 ng mL-1 and 1.9 ng mL-1, respectively. The relative standard deviation for the seven analyses was 7.1%. The relative recoveries of vitamin D3 in spiked human serum samples were between 85% and 97%. The amount of 25-OH-vitamin D3 in samples was determined using the proposed method and acceptable results were reported.
This article was published in the following journal.
Name: Journal of chromatographic science
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Miniaturized methods of liquid-liquid extraction.
The removal of a soluble component from a liquid mixture by contact with a second liquid, immiscible with the carrier liquid, in which the component is preferentially soluble. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A method of separation of two or more substances by repeated distribution between two immiscible liquid phases that move past each other in opposite directions. It is a form of liquid-liquid chromatography. (Stedman, 25th ed)
Chromatographic techniques in which the mobile phase is a liquid.
Materials in intermediate state between solid and liquid.
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...