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Cone beam X-ray luminescence computed tomography (CB-XLCT) is a promising imaging technique in studying the physiological and pathological processes in small animals. However, the dynamic bio-distributions of probes in small animal especially in adjacent targets are still hard to be captured directly from dynamic CB-XLCT. In this work, a 4D temporal-spatial reconstruction method based on principal component analysis (PCA) in the projection space is proposed for dynamic CB-XLCT. Firstly, projections of angles in each 3D frame are compressed to reduce the noises initially. Then a temporal PCA is performed on the projection data to decorrelate the 4D problem into separate 3D problems in the PCA domain. In the PCA domain, the difference between dynamic behaviors of multiple targets can be reflected by the first several principal components which can be further used for fast and improved reconstruction by a restarted Tikhonov regularization method. At last, by discarding the principal components mainly reflecting noise, the concentration series of targets are recovered from the first few reconstruction results with a mask as the constraint. Numerical simulation and phantom experiment demonstrate that the proposed method can resolve multiple targets and recover the dynamic distributions with high computation efficiency. The proposed method provides a new feasibility for imaging dynamic bio-distributions of probes in vivo.
This article was published in the following journal.
Name: IEEE transactions on medical imaging
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Modality of computed tomography in which the patient is irradiated in a spiral path around the body with a cone or pyramid-shaped beam.
Computed tomography modalities which use a cone or pyramid-shaped beam of radiation.
Photosensitive proteins expressed in the CONE PHOTORECEPTOR CELLS. They are the protein components of cone photopigments. Cone opsins are classified by their peak absorption wavelengths.
A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The science and application of a double-beam transmission interference microscope in which the illuminating light beam is split into two paths. One beam passes through the specimen while the other beam reflects off a reference mirror before joining and interfering with the other. The observed optical path difference between the two beams can be measured and used to discriminate minute differences in thickness and refraction of non-stained transparent specimens, such as living cells in culture.
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