Advertisement

Topics

Microbial biomass turnover times and clues to cellular protein repair in energy-limited deep Baltic Sea sediments.

08:00 EDT 16th May 2019 | BioPortfolio

Summary of "Microbial biomass turnover times and clues to cellular protein repair in energy-limited deep Baltic Sea sediments."

The discovery of active microbial life deeply buried beneath the seafloor has opened important questions: how do microorganisms cope with extreme energy limitation, what is their metabolic activity, and how do they repair damages to essential biomolecules? We used a
D:
L-amino acid model to calculate microbial biomass turnover times. We used a metagenome and metatranscriptome analysis to investigate the distribution of the gene that encodes Protein-L-iso aspartate(D-aspartate) O-methyltransferase (PCMT), an enzyme which recognizes damaged L-isoapartyl and D-aspartyl residues in proteins and catalyzes their repair. Sediment was retrieved during the Integrated Ocean Drilling Program (IODP) Expedition 347 from Landsort Deep and the Little Belt in the Baltic Sea. The study covers the period from the Baltic Ice Lake ca. 13,000 years ago to the present. Our results provide new knowledge on microbial biomass turnover times and protein repair in relation to different regimes of organic matter input. For the first time, we show that the PCMT gene was widely distributed and expressed among phylogenetically diverse groups of microorganisms. Our findings suggest that microbial communities are capable of repairing D-amino acids within proteins using energy obtained from the degradation of a mixture of labile compounds in microbial necromass and more recalcitrant organic matter.

Affiliation

Journal Details

This article was published in the following journal.

Name: FEMS microbiology ecology
ISSN: 1574-6941
Pages:

Links

DeepDyve research library

PubMed Articles [23201 Associated PubMed Articles listed on BioPortfolio]

Rhizosphere effects on soil microbial community structure and enzyme activity in a successional subtropical forest.

Forest succession is a central ecological topic due to the importance of its dynamic process for terrestrial ecosystems. However, we have limited knowledge of the relationship between forest successio...

Autophagy accounts for approximately one-third of mitochondrial protein turnover and is protein selective.

The destruction of mitochondria through macroautophagy/autophagy has been recognized as a major route of mitochondrial protein degradation since its discovery more than fifty years ago, but fundamenta...

Effect of the amount of organic trigger compounds, nitrogen and soil microbial biomass on the magnitude of priming of soil organic matter.

Priming effects (PEs) are defined as short-term changes in the turnover of soil organic matter (SOM) caused by the addition of easily degradable organic compounds to the soil. PEs are ubiquitous but t...

A new perspective on oxidation of DNA repair proteins and cancer.

Reactive oxygen and nitrogen species (RONS) are formed as byproducts of many endogenous cellular processes, in response to infections, and upon exposure to various environmental factors. An increase i...

Uptake of phosphate by Synechocystis sp. PCC 6803 in dark conditions: Removal driving force and modeling.

Rapid uptake of inorganic phosphate (Pi) by microalgae should occur through two processes operating in parallel: onto extracellular polymeric substances (EPS) and intracellular polymeric substances (I...

Clinical Trials [6489 Associated Clinical Trials listed on BioPortfolio]

Specialized Food Plan Based on Individual Physiological Comprehensive Body Assessments Accompanied With Cellular Repair Therapy to Decrease Inflammation Cognitively Impaired Patients

Diet plays a large role in inflammation, oxidative stress and cognition; however, every person's body type, resting metabolic rate, BMI, and inflammation levels vary. Through performing ph...

CLA and Vitamin D on Protein Turnover

To conduct a randomized, double-blind, controlled clinical trial to determine the independent and combined effects of dietary conjugated linoleic acid (CLA) and vitamin D supplementation o...

Clinical and Microbial Efficacy of ISV403 in Bacterial Conjunctivitis

To evaluate the clinical and microbial efficacy of ISV-403 administered three times a day for 5 days compared to vehicle three times a day for 5 days in the treatment of bacterial conjunct...

Androgen Deprivation Therapy Muscle Protein Metabolism and Blood Glucose

Prostate cancer (PCa) patients receiving androgen deprivation therapy (ADT) (e.g., Zoladex), experience troublesome side effects during and after treatment (e.g., loss of lean body mass (L...

Efficacy in Walked Distance of Indacaterol vs Tiotropium in Women With COPD Secondary to Biomass (EMERALD)

Chronic Obstructive Pulmonary Disease (COPD) secondary to biomass exposure constitutes a chronic respiratory condition frequently excluded from large clinical trials. Biomass exposure COPD...

Medical and Biotech [MESH] Definitions

Light absorbing proteins and protein prosthetic groups found in certain microorganisms. Some microbial photoreceptors initiate specific chemical reactions which signal a change in the environment, while others generate energy by pumping specific ions across a cellular membrane.

A DNA-binding protein that mediates DNA REPAIR of double strand break, and GENETIC RECOMBINATION.

An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.

Disorders resulting from defective DNA REPAIR processes or the associated cellular responses to DNA DAMAGE.

DNA repair proteins that include the bacterial MutL protein and its eukaryotic homologs. They consist of a conserved N-terminal region with weak ATPase activity, an endonuclease motif, and a C-terminal domain that forms MutL homodimers or heterodimers between MLH1 and the PMS1, MISMATCH REPAIR ENDONUCLEASE PMS2; or MLH3 proteins. These complexes function in DNA repair pathways, primarily DNA MISMATCH REPAIR, where MutL/MLH1 and the MUTS DNA MISMATCH-BINDING PROTEIN are targeted to damaged DNA.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


Searches Linking to this Article