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This chapter gives examples of basic procedures of quantification of plant structures with use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified "rules of thumb" are presented. The basic procedures mentioned in the text are: (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape.
This article was published in the following journal.
Name: Methods in molecular biology (Clifton, N.J.)
Due to low labeling efficiency and structural heterogeneity in fluorescence-based single-molecule localization microscopy (SMLM), image alignment and quantitative analysis is often required to make ac...
This Statistical Analysis Plan (SAP) details the statistical procedures to be applied for the analysis of data for the multi-center electroencephalography (EEG) study. It consists of a basic descripti...
This protocol describes the automated imaging and a quantitative analysis of the morphology of small plants from the moss Physcomitrella patens. This method can be used for the analysis of growth phen...
We introduce Delaunay Point Processes, a framework for the extraction of geometric structures from images. Our approach simultaneously locates and groups geometric primitives (line segments, triangles...
Machine learning for ultrasound image analysis and interpretation can be helpful in automated image classification in large-scale retrospective analyses to objectively derive new indicators of abnorma...
This study aims to explore whether the increased supply of dietary plant sterols and plant stanols have any influence on serum levels of phytosterols and on consistency of carotid atheroma...
The aim is to investigate the effects of yoghurt drinks containing two doses of plant stanol ester either with or without added camelina oil on the serum cholesterol levels in moderately h...
RATIONALE: Diagnostic procedures, such as digital image analysis and fluorescent in situ hybridization, may help doctors learn the extent of Barrett esophagus. PURPOSE: This randomized ph...
The primary aim of the study is to investigate whether optical coherence tomography (OCT) may be a useful tool for investigating the in-vivo histology of ocular structures in patients with...
The effect of a diet free from animal-sourced nutrients on body composition in weight reduction programs is not well established. In this non-randomised free living interventional case-con...
The description and measurement of the various factors that produce physical stress upon dental restorations, prostheses, or appliances, materials associated with them, or the natural oral structures.
X-ray image-detecting devices that make a focused image of body structures lying in a predetermined plane from which more complex images are computed.
A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.
A philosophical description of intrinsic self-worth and dignity which bestows basic rights of life, liberty, and freedom from harm.
Description of pattern of recurrent functions or procedures frequently found in organizational processes, such as notification, decision, and action.