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Biotechnological production of ruscogenins in plant cell and organ cultures of Ruscus aculeatus.

08:00 EDT 29th May 2019 | BioPortfolio

Summary of "Biotechnological production of ruscogenins in plant cell and organ cultures of Ruscus aculeatus."

Ruscus aculeatus is a threatened medicinal plant whose main bioactive components, the ruscogenins, have long been used in the treatment of hemorrhoids and varicose veins, but recently demonstrated activity against some types of cancer. Plant cell biofactories could constitute an alternative to the whole plant as a source of ruscogenins. In this pipeline, despite the in vitro recalcitrance of R. aculeatus, after many attempts we developed friable calli and derived plant cell suspensions, and their ruscogenin production was compared with that of organized in vitro plantlet and root-rhizome cultures. Root-rhizomes showed a higher capacity for biomass and ruscogenin production than the cell suspensions and the yields were greatly improved by elicitation with coronatine. Although ruscogenins accumulate in plants mainly in the root-rhizome, it was demonstrated that the aerial part could play an important role in their biosynthesis, as production was higher in the whole plant than in the root-rhizome cultures.

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This article was published in the following journal.

Name: Plant physiology and biochemistry : PPB
ISSN: 1873-2690
Pages: 133-141

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Medical and Biotech [MESH] Definitions

A technique for maintaining or growing CELLS in vitro. Cultures of dispersed cells derived directly from fresh TISSUES are called primary cell cultures. Cultures may also derive from established CELL LINE usually stored frozen.

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.

A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)

Established cell cultures that have the potential to propagate indefinitely.

A plant genus of the family LILIACEAE. This should not be confused with Broom or Scotch Broom (CYTISUS) or Desert Broom (BACCHARIS) or Spanish Broom (SPARTIUM) or Brome (BROMUS).

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