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Nearly monodisperse Zinc oxide (ZnO) quantum dots (QDs) displayed yellow fluorescence were synthesized using urea as dispersant. In this paper, urea-ZnO QDs were used as fluorescent probe to detect Cr6+ in solution. The emission from the as-synthesized urea-ZnO QDs is selectively quenched when Cr6+ ions were added. Moreover, there are two linear relationships between the quenching of fluorescence intensity and the Cr6+ concentrations ranging from 4 μM to 1000 μM, with the detection limit for Cr6+ at 19.53 nM (on basis of 3σ/slope criterion). The quenching of fluorescence is attributed to aggregation of the QDs and charge transfer between the QDs and Cr6+ by measurements of transmission electron microscopy (TEM) images, UV-visible absorption spectra and fluorescence lifetime.
This article was published in the following journal.
Name: Methods and applications in fluorescence
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Nanometer sized fragments (the dots) of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They are brighter and more persistent than organic chemical INDICATORS. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY.
Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.
A form of fluorescent antibody technique utilizing a fluorochrome conjugated to an antibody, which is added directly to a tissue or cell suspension for the detection of a specific antigen. (Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
A nonfluorescent reagent for the detection of primary amines, peptides and proteins. The reaction products are highly fluorescent.
The urea concentration of the blood stated in terms of nitrogen content. Serum (plasma) urea nitrogen is approximately 12% higher than blood urea nitrogen concentration because of the greater protein content of red blood cells. Increases in blood or serum urea nitrogen are referred to as azotemia and may have prerenal, renal, or postrenal causes. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)