Peroxidase mimetic activity of porphyrin modified ZnFeO/reduced graphene oxide and its application for colorimetric detection of HO and glutathione.

08:00 EDT 5th June 2019 | BioPortfolio

Summary of "Peroxidase mimetic activity of porphyrin modified ZnFeO/reduced graphene oxide and its application for colorimetric detection of HO and glutathione."

Artificial nanoenzymes which can overcome some drawbacks of natural enzymes is a challenging topic in the biosensor field. Herein, we demonstrated 5, 10, 15, 20-tetrakis (4-carboxylpheyl)-porphyrin modified magnetic ZnFeO nanoparticles loaded on the surface of reduced graphene oxide (Por-ZnFeO/rGO), which exhibited intrinsic peroxidase-like activity and rapidly oxidized the peroxidase substrate 3, 3', 5, 5'-tetramethylbenzidine (TMB) into a blue product (OxTMB) distinguished by naked eyes. Interestingly, by comparative study of different nanomaterials ZnFeO nanoparticles, ZnFeO/rGO and Por-ZnFeO, Por-ZnFeO/rGO was proved to possess the highest peroxidase-like activity. Electron spin resonance (ESR) verified the catalytic activity of Por-ZnFeO/rGO for HO was due to hydroxyl radical from decomposition of HO. Temperature and pH strongly affected the peroxidase-like activity of Por-ZnFeO/rGO nanocomposites. Under optimal conditions (pH = 4, 40 °C), the constructed sensor based on the catalytic activity of the Por-ZnFeO/rGO could be conveniently used for colorimetric detection of HO in the range of 0.7-30 μM with the detection limit of 0.54 μM. Moreover, the colorimetric sensor based on Por-ZnFeO/rGO exhibited a good linear response to glutathione (GHS) in the range of 2-40 μM with a low detection limit of 0.76 μM. The detection of GHS can be easily realized through the obvious color change by naked eyes without any complicated instrumentation.


Journal Details

This article was published in the following journal.

Name: Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Pages: 567-575


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