Coaxial sensing bio-amplifier for ultrasensitive detections of circulating tumor DNAs.

08:00 EDT 5th June 2019 | BioPortfolio

Summary of "Coaxial sensing bio-amplifier for ultrasensitive detections of circulating tumor DNAs."

We herein report the first attempt to engineer a coaxial-sensing 3D amplifier able to achieve dynamic self-assembly in response to a mutated-ctDNA target. A bio-nanofiber is firstly manufactured via an ingenious double-channel electrostatic spinning and DNA rolling circle replication (RCR) technology, which offered an ideal scaffold for assembly of 3D amplifier activated by target recognition. The coaxial-controllable signal amplifier presented several advantages. (1) Given its "coaxial sensing effect", the proposed bio-amplifier played the coaxial transduction for signal enrichment to vastly increase sensitivity, capable of discriminating a single-base mismatched sequence from the perfectly complementary one, using ctDNA-134A as a model analyte. (2) Due to "covalent bridges lock effect" in an identifying chip with locked nucleic acid beacons, this 3D amplifier expressed high specificity and biostability toward seven different mutated-ctDNAs. (3) Profiting from special configuration of bioactive nanofibers and DNA replication programming, this catalytic bio-amplifier possessed "signal enrichment effect", which enhanced dynamic range toward ctDNA-134A detection and hybridized without any external indicators. This innovative bio-amplifier has a detection limit of 5.1 aM for ctDNA-134A with superior specificity, excellent sensitivity, and good performance. This pioneered method was further applied for broadly differentiate cells and evaluate changes in the expression levels of intracellular mutated-ctDNAs.


Journal Details

This article was published in the following journal.

Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages: 111414


DeepDyve research library

PubMed Articles [9291 Associated PubMed Articles listed on BioPortfolio]

Ultrasensitive SERS detection of nucleic acids via simultaneous amplification of target-triggered enzyme-free recycling and multiple-reporter.

The development of ultrasensitive and specific methods for facile detection of trace nucleic acids is of great significance to human health and safety. In the present work, an ultrasensitive SERS-base...

Superwettable colloidal crystal micropatterns on butterfly wing surface for ultrasensitive detection.

Ultrasensitive detections with enrichment approaches based on hydrophilic-hydrophobic patterns have attracted increasing attention in the early diagnosis and treatment of diseases. However, most of th...

Target capturing performance of microfluidic channel surface immobilized aptamers: the effects of spacer lengths.

Aptamers have been widely used to recognize and capture their targets in sensitive detection applications, such as in detections of circulating tumor cells. In this study, we investigate the effects o...

Ultrasensitive DNA biosensor based on electrochemical atom transfer radical polymerization.

Here we report a highly selective and ultrasensitive DNA biosensor based on electrochemical atom transfer radical polymerization (ATRP) signal amplification and "Click Chemistry". The DNA biosensor wa...

High-activity FeO nanozyme as signal amplifier: A simple, low-cost but efficient strategy for ultrasensitive photoelectrochemical immunoassay.

Sensitive but with simple, inexpensive detection of disease-related biomarkers in real biological samples is of quite necessity for early diagnosis and disease surveillance. We herein first introduced...

Clinical Trials [6260 Associated Clinical Trials listed on BioPortfolio]

HBV-host cfDNA as Minimal Residual Tumor Marker for HBV-related HCC

Early stage HCC is treated by curative surgical resection or by local ablation (such as radio-frequency) as the current standard of care. The complete removal of clinical visible HCC is th...

A Pilot Study for the Evaluation of the Safety and Performance of a Combined OCT System

The novel Coaxial Optical Coherence Tomography (OCT) System is designed to visualize both structures of the retina and choroid. The purpose of this research is the safety and evaluation o...

Dual Wavelength OCT

The purpose of this research is the evaluation of a combined coaxial optical coherence tomography (OCT) system to image retina/choroid and to evaluate if post processing of the data can gi...

Dynamic Changes of Circulating Tumor DNA in Surgical Lung Cancer Patients

Previous study showed circulating tumor DNA levels reflect the total systemic tumor burden. Circulating tumor DNA levels should decrease after complete surgery and could be increase as tum...

Renal Parenchymal Core Needle Biopsy

Percutaneous image-guided parenchymal renal biopsy has been used to detect the different pathologies of renal parenchyma, to define the degree of reversible changes, and to define when the...

Medical and Biotech [MESH] Definitions

DNA released from tumor cells that is found circulating in PLASMA; SERUM; or other BODY FLUIDS.

A class of G-protein-coupled receptors that react to varying extracellular CALCIUM levels. Calcium-sensing receptors in the PARATHYROID GLANDS play an important role in the maintenance of calcium HOMEOSTASIS by regulating the release of PARATHYROID HORMONE. They differ from INTRACELLULAR CALCIUM-SENSING PROTEINS which sense intracellular calcium levels.

DNA that is complementary to the sense strand. (The sense strand has the same sequence as the mRNA transcript. The antisense strand is the template for mRNA synthesis.) Synthetic antisense DNAs are used to hybridize to complementary sequences in target RNAs or DNAs to effect the functioning of specific genes for investigative or therapeutic purposes.

Process whereby a cell, bodily structure, or organism (animal or plant) receives or detects a gravity stimulus. Gravity sensing plays an important role in the directional growth and development of an organism (GRAVITROPISM).

Intracellular signaling peptides and proteins that bind to CALCIUM. They undergo allosteric changes when bound to CALCIUM that affects their interaction with other signal-transducing molecules. They differ from CALCIUM-SENSING RECEPTORS which sense extracellular calcium levels.

Quick Search


DeepDyve research library

Relevant Topics

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Complementary and Alternative Medicine
Alternative medicine are whole medical systems that did not fit with conventional medicine as they have completely different philosophies and ideas on the causes of disease, methods of diagnosis and approaches to treatment. Although often overlapping, co...

Searches Linking to this Article