Advertisement

Topics

The cytoplasmic portion of the T3SS inner membrane ring components sort into distinct families based on biophysical properties.

08:00 EDT 10th June 2019 | BioPortfolio

Summary of "The cytoplasmic portion of the T3SS inner membrane ring components sort into distinct families based on biophysical properties."

Type III secretion systems are used by many Gram-negative bacteria to inject effector proteins into eukaryotic cells to subvert their normal activities. Structurally conserved portions of the type III secretion apparatus include a: basal body located within the bacterial envelope; an exposed needle with tip complex that delivers effectors across the target cell membrane; and cytoplasmic sorting platform that selects cargo and powers secretion. While structurally conserved, the individual proteins that make up this nanomachine are typically not interchangeable though they do tend to fall into families. Here we selected a single domain from the inner membrane ring of the basal body from six different type III secretion systems (called SctD using a proposed unifying nomenclature). The selected domain creates an integral interface between the basal body and the sorting platform. Therefore, it represents a pivotal point between two distinct assemblies. All six protein domains possess a structural motif called a forkhead-associated-like (FHA-like) domain but differ greatly in their sequences and solution behaviors. These differences are used here to define family boundaries for these FHA-like domains. The data parallel, though not precisely, family boundaries defined by other proteins within the apparatus and by phylogenetic analysis. Ultimately, differences in the families are likely to reflect differences in the activities of these type III secretion systems or the host niches in which these pathogens are found.

Affiliation

Journal Details

This article was published in the following journal.

Name: Biochimica et biophysica acta. Proteins and proteomics
ISSN: 1878-1454
Pages:

Links

DeepDyve research library

PubMed Articles [6675 Associated PubMed Articles listed on BioPortfolio]

Transport of organic substances through the cytoplasmic membrane of cyanobacteria.

Cyanobacteria are mainly known to incorporate inorganic molecules like carbon dioxide and ammonia from the environment into organic material within the cell. Nevertheless cyanobacteria do import and e...

Reconstituting Autophagy Initiation from Purified Components.

The hallmark of macroautophagy is the de novo generation of a membrane structure that collects cytoplasmic material and delivers it to lysosomes for degradation. The nucleation of this precursor membr...

Mg2+ homeostasis and transport in cyanobacteria - at the crossroad of bacterial and chloroplast Mg2+ import.

Mg2+ is the most abundant divalent cation in living cells, where it is required for various intracellular functions. In chloroplasts and cyanobacteria, established photosynthetic model systems, Mg2+ i...

Insights into water accessible pathways and the inactivation mechanism of proton translocation by the membrane-embedded domain of V-type ATPases.

V-type ATPases are multi-protein complexes, which acidify cellular compartments in eukaryotes. They pump protons against an ion gradient, driven by a mechano-chemical framework that exploits ATP hydro...

Pattern recognition receptors and their interactions with bacterial type III effectors in plants.

Innate immune signaling of plants is initiated by pattern recognition receptors (PRRs) at the plasma membrane. Upon pathogen attack, PRRs recognize pathogen-associated molecular patterns (PAMPs) via e...

Clinical Trials [1608 Associated Clinical Trials listed on BioPortfolio]

Performance and Safety of Vaginal Ring Applicator in Healthy Females (MK-8342A-063)

The purpose of the study is to assess the performance and safe use of the vaginal ring applicator as an optional aid for insertion of a placebo vaginal ring into the vagina in healthy wome...

The Influence of Satiation Measures on the Portion Size Effect

The purpose of this study is to gain a better understanding of the satiation measures that influence human eating behavior in regard to intake of large portion sizes at a meal. The investi...

Efficacy and Safety of Etonogestrel + 17β-Estradiol Vaginal Ring in Treatment of Women With Primary Dysmenorrhea (MK-8342B-060)

The purpose of this study is to evaluate the efficacy of the etonogestrel (ENG) + 17β-estradiol (E2) vaginal ring compared to placebo vaginal ring in the treatment of dysmenorrhea at Trea...

A Trial In Healthy HIV-Negative Women To Assess The Effect Of Menses And Tampon Use On The Pharmacokinetics Of Dapivirine, Delivered By Dapivirine Vaginal Ring-004, Containing 25 Mg Of Dapivirine

A Two-Cohort, Open-Label, Randomised, Three-Period Crossover Trial In Healthy HIV-Negative Women To Assess The Effect Of Menses And Tampon Use On The Pharmacokinetics Of Dapivirine, Delive...

A Study to Evaluate the Effect of Vaginal Rings on Primary Dysmenorrhea (P08257/MK-8175A/MK-8342B-057)

The study investigates four vaginal ring groups with respect to relief of primary dysmenorrhea, and should identify at least one vaginal ring that shows clinically relevant treatment effic...

Medical and Biotech [MESH] Definitions

Components of the cytoplasm excluding the CYTOSOL.

A ring of tissue extending from the scleral spur to the ora serrata of the retina. It consists of the uveal portion and the epithelial portion. The ciliary muscle is in the uveal portion and the ciliary processes are in the epithelial portion.

Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.

Functionally and structurally differentiated, purple-pigmented regions of the cytoplasmic membrane of some strains of Halobacterium halobium. The membrane develops under anaerobic conditions and is made almost entirely of the purple pigment BACTERIORHODOPSINS. (From Singleton & Sainsbury Dictionary of Microbiology and Molecular Biology, 2d ed)

A network of membrane compartments, located at the cytoplasmic side of the GOLGI APPARATUS, where proteins and lipids are sorted for transport to various locations in the cell or cell membrane.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Biosimilars
Biosimilars or Follow-on biologics are terms used to describe officially approved subsequent versions of innovator biopharmaceutical products made by a different sponsor following patent and exclusivity expiry on the innovator product. Products that ar...

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Article