Advertisement

Topics

Membrane action of polyhexamethylene guanidine hydrochloride revealed on smooth muscle cells, nerve tissue and rat blood platelets: A biocide driven pore-formation in phospholipid bilayers.

08:00 EDT 10th June 2019 | BioPortfolio

Summary of "Membrane action of polyhexamethylene guanidine hydrochloride revealed on smooth muscle cells, nerve tissue and rat blood platelets: A biocide driven pore-formation in phospholipid bilayers."

A well-known cationic biocide of guanidine polymer family, polyhexamethylene guanidine hydrochloride (PHMG) has been tested against smooth muscle cells isolated from swine myometrium, synaptosomes of rat brain nerve terminals and rat blood platelets for the membrane action. It was established that PHMG blocked the activity of Na,K-ATPase of smooth muscle cells plasma membrane by 82.2 ± 0.9% at a concentration of 7 ppm, whilst a dose-dependent depolarization of synaptosomes and platelets became appreciable at 100-500 ppm. Comparative studies by the methods of mass spectrometry (MALDI-TOF and PDMS-TOF), viscosimetry, dynamic light scattering and model phospholipid membranes revealed PHMG oligomers with various number of repeat units (8-16) that formed K-selective potential-dependent pores in sterol-free phosphatidylethanolamine-containing phospholipid bilayers at a concentration of 1 ppm. Obtained results suggest that besides acidic lipids and membrane proteins phosphatidylethanolamine and cholesterol are the other major factors responsible for the differences between PHMG-induced plasma membrane depolarization of microbial and eukaryotic cells and thus, diverse modes of PHMG membrane action.

Affiliation

Journal Details

This article was published in the following journal.

Name: Toxicology in vitro : an international journal published in association with BIBRA
ISSN: 1879-3177
Pages:

Links

DeepDyve research library

PubMed Articles [14711 Associated PubMed Articles listed on BioPortfolio]

Expression of a Functional IL-2 Receptor in Vascular Smooth Muscle Cells.

Many nonlymphoid cell types express at least two, if not all three, subunits of the IL-2R; although, compared with lymphocytes, relatively little is known about how IL-2 affects the function of nonlym...

Double deletion of calponin 1 and calponin 2 in mice decreases systemic blood pressure with blunted length-tension response of aortic smooth muscle.

Calponin is a family of actin filament-associated regulatory proteins. Among its three isoforms, calponin 1 is smooth muscle specific and calponin 2 is expressed in smooth muscle and certain non-muscl...

Mitochondrial regulation of airway smooth muscle functions in health and pulmonary diseases.

Mitochondria are important for airway smooth muscle physiology due to their diverse yet interconnected roles in calcium handling, redox regulation, and cellular bioenergetics. Increasing evidence indi...

Conditional deletion of smooth muscle Cullin-3 causes severe progressive hypertension.

Patients with mutations in Cullin-3 (CUL3) exhibit severe early onset hypertension but the contribution of the smooth muscle remains unclear. Conditional genetic ablation of CUL3 in vascular smooth mu...

Effect of polyhexamethylene biguanide functionalized silver nanoparticles on the growth of Staphylococcus aureus.

Polyhexamethylene biguanide (PHMB) was used as a stabilizing ligand to synthesize uniform silver nanoparticles (Ag NPs). The effects and action mechanism of PHMB functionalized Ag NPs (Ag NPs-PHMB) on...

Clinical Trials [7626 Associated Clinical Trials listed on BioPortfolio]

Modulation of Vascular Calcification in Chronic Dialysis Patients

48 patients will be randomized and treated with MCO or highflux dialysis for six months (24 weeks) after a run-in phase of 4 weeks Highflux treatment. Serum samples will be drawn at basel...

Sorting and Expression Profiling of Airway Cells From Humans (The SEARCH Study)

This will be a single site, mechanistic study of asthmatic subjects and healthy, non-asthmatic controls which will compare asthmatic smooth muscle cells to non-asthmatic smooth muscle cell...

Airway Remodeling During Mepolizumab Treatment

Chronic airway changes, such as smooth muscle hypertrophy/hyperplasia, reticular basement membrane (RBM) thickening, goblet cells hyperplasia characterize severe asthma. Chronic inflammati...

Fatty Acids in Airway Smooth Muscle of Asthmatics

In this proof of concept study, we aim to determine if supplementation with coconut oil causes an increase in cholecystokinin and cholecystokinin receptor expression in the airway smooth m...

Sildenafil and Outcome of IVF/ICSI Cycles

Adequate growth of the endometrium is crucial for implantation. Accordingly, the pregnancy success rate of patients with a thin endometrium is low. Furthermore, there is a little informati...

Medical and Biotech [MESH] Definitions

A class I anti-arrhythmic agent (one that interferes directly with the depolarization of the cardiac membrane and thus serves as a membrane-stabilizing agent) with a depressant action on the heart similar to that of guanidine. It also possesses some anticholinergic and local anesthetic properties.

Precursor cells destined to differentiate into smooth muscle myocytes (MYOCYTES, SMOOTH MUSCLE).

A tumor composed of smooth muscle tissue, as opposed to leiomyoma, a tumor derived from smooth muscle.

A galectin found abundantly in smooth muscle (MUSCLE, SMOOTH) and SKELETAL MUSCLE and many other tissues. It occurs as a homodimer with two 14-kDa subunits.

Mature contractile cells, commonly known as myocytes, that form one of three kinds of muscle. The three types of muscle cells are skeletal (MUSCLE FIBERS, SKELETAL), cardiac (MYOCYTES, CARDIAC), and smooth (MYOCYTES, SMOOTH MUSCLE). They are derived from embryonic (precursor) muscle cells called MYOBLASTS.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Rheumatology
Arthritis Fibromyalgia Gout Lupus Rheumatic Rheumatology is the medical specialty concerned with the diagnosis and management of disease involving joints, tendons, muscles, ligaments and associated structures (Oxford Medical Diction...

Blood
Blood is a specialized bodily fluid that delivers necessary substances to the body's cells (in animals) – such as nutrients and oxygen – and transports waste products away from those same cells.  In vertebrates, it is composed of blo...


Searches Linking to this Article