Comparative proteomics reveals concordant and discordant biochemical effects of caffeine versus epigallocatechin-3-gallate in human endothelial cells.

08:00 EDT 10th June 2019 | BioPortfolio

Summary of "Comparative proteomics reveals concordant and discordant biochemical effects of caffeine versus epigallocatechin-3-gallate in human endothelial cells."

Caffeine and epigallocatechin-3-gallate (EGCG) are the most abundant bioactive chemicals found in coffee and tea, respectively. While they are known to regulate normal physiology and body homeostasis, their biochemical effects, particularly at cellular and subcellular levels, remain under-investigated. We thus performed comparative proteomics study followed by bioinformatics analyses to investigate differential biochemical effects of these two chemicals on human endothelial cells. EA.hy926 cells were incubated with 100 μM caffeine or EGCG for 24-h and then subjected to label-free quantitative proteomics using nanoLC-ESI-Qq-TOF MS/MS compared to the control cells. A total of 142 and 152 significantly altered proteins were identified in caffeine-exposed and EGCG-exposed cells, respectively. Among these, 86 were the common changes found in both caffeine-exposed and EGCG-exposed cells. Bioinformatics revealed that both caffeine and EGCG mostly affected ribosomal proteins involving protein synthesis for membrane destination and secretion (e.g., SRP-dependent cotranslational protein targeting to membrane, protein targeting to endoplasmic reticulum, nuclear-transcribed mRNA catabolic process, nonsense-mediated decay, etc.). While the down-regulated proteins were similar in both groups, the up-regulated proteins in the EGCG-exposed cells highlighted the promoting effects of EGCG on actin-crosslink formation, glycolysis and ubiquitin-proteasome activity. These concordant and discordant changes in cellular proteome of human endothelial cells induced by caffeine and EGCG are useful for better understanding of biochemical/physiological effects of these two bioactive chemicals.


Journal Details

This article was published in the following journal.

Name: Toxicology and applied pharmacology
ISSN: 1096-0333
Pages: 114621


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Medical and Biotech [MESH] Definitions

A methylxanthine naturally occurring in some beverages and also used as a pharmacological agent. Caffeine's most notable pharmacological effect is as a central nervous system stimulant, increasing alertness and producing agitation. It also relaxes SMOOTH MUSCLE, stimulates CARDIAC MUSCLE, stimulates DIURESIS, and appears to be useful in the treatment of some types of headache. Several cellular actions of caffeine have been observed, but it is not entirely clear how each contributes to its pharmacological profile. Among the most important are inhibition of cyclic nucleotide PHOSPHODIESTERASES, antagonism of ADENOSINE RECEPTORS, and modulation of intracellular calcium handling.

The systematic study of the complete complement of proteins (PROTEOME) of organisms.

A purine base found in most body tissues and fluids, certain plants, and some urinary calculi. It is an intermediate in the degradation of adenosine monophosphate to uric acid, being formed by oxidation of hypoxanthine. The methylated xanthine compounds caffeine, theobromine, and theophylline and their derivatives are used in medicine for their bronchodilator effects. (Dorland, 28th ed)

A class of cell surface receptors that prefers ADENOSINE to other endogenous purines. Purinergic P1 receptors are widespread in the body including the cardiovascular, respiratory, immune, and nervous systems. There are at least two pharmacologically distinguishable types (A1 and A2, or Ri and Ra). The methylxanthines, e.g., CAFFEINE, bind to these receptors, but also have other unrelated effects.

The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

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