Advertisement

Topics

Apolipoprotein E and periostin are potential biomarkers of nasal mucosal inflammation: a parallel approach of and secretomes.

08:00 EDT 13th June 2019 | BioPortfolio

Summary of "Apolipoprotein E and periostin are potential biomarkers of nasal mucosal inflammation: a parallel approach of and secretomes."

No previously suggested biomarkers of nasal mucosal inflammation have been practically applied in clinical fields, and nasal epithelium-derived secreted proteins as biomarkers have not specifically been investigated. The goal of this study was to identify secreted proteins that dynamically change during the differentiation from basal cells to fully differentiated cells and examine whether nasal epithelium-derived proteins can be used as biomarkers of nasal mucosal inflammation, such as chronic rhinosinusitis. To achieve this goal, we analyzed two secretomes using the isobaric tag for relative and absolute quantification (iTRAQ) technique. From in vitro secretomes, we identified the proteins altered in apical secretions of primary human nasal epithelial cells (HNECs) according to the degree of differentiation; from in vivo secretomes, we identified the increased proteins in nasal lavage (NAL) fluids obtained from patients 2 weeks after endoscopic sinus surgery for chronic sinusitis. We then used a parallel approach to identify specific biomarkers of nasal mucosal inflammation; first, we selected apolipoprotein E (APOE) as a nasal epithelial cell-derived biomarker through screening proteins that were upregulated in both in vitro and in vivo secretomes, and verified highly secreted APOE in NAL fluids of the patients by Western blotting. Next, we selected periostin (POSTN) as an inflammatory mediator-inducible biomarker from in vivo secretomes, whose secretion was not induced under in vitro culture condition. We demonstrated that those two nasal epithelium-derived proteins are possible biomarkers of nasal mucosal inflammation.

Affiliation

Journal Details

This article was published in the following journal.

Name: American journal of respiratory cell and molecular biology
ISSN: 1535-4989
Pages:

Links

DeepDyve research library

PubMed Articles [16235 Associated PubMed Articles listed on BioPortfolio]

Periostin: An emerging biomarker for allergic diseases.

Periostin is a matricellular protein as well as an extracellular matrix (ECM) protein belonging to the fasciclin family. Periostin plays important roles as a matricellular protein in the setting of al...

Regulation of KDM2B and Brg1 on Inflammatory Response of Nasal Mucosa in CRSwNP.

Chronic nasal sinusitis with nasal polyps (CRSwNP) is a reversible nasal mucosal remodeling disease caused by persistent inflammation and structural changes in chronic nasal mucosa. Although there hav...

Nasal discharge in dogs - are microbiological and histopathological examinations clinically useful?

Retrospective evaluation of the diagnostic value of bacterial culture of nasal mucosal swabs and histopathologic assessment of nasal mucosal biopsies in dogs with nasal discharge.

Nasalleukoplakia:update on diagnosis and management.

Nasal leukoplakia, defined as nasal mucosal grayish white lesion accompanied by adjacent mucosa thickening and hyperemia, is a kind of precancerous lesion. Since a case of nasal septum mucosal leukopl...

Effect of inhaled corticosteroids on serum periostin levels in adult patients with mild-moderate asthma.

Periostin is an extracellular matrix protein, and its expression is upregulated in airway epithelial cells in patients with bronchial asthma. Periostin may be a key molecule linked to type 2 T-helper ...

Clinical Trials [10250 Associated Clinical Trials listed on BioPortfolio]

BIOmarkers in Severe AsthMa Patients on Omalizumab Treatment

Eosinophil infiltration and degranulation in airways has been implicated in the pathology of asthma. Periostin is considered to be a marker of eosinophilic inflammation and is one of the h...

The Correlation of Periostin and Renal Pathology in Chronic Kidney Disease Patients

The purpose of this study is to determine the location of periostin and urine periostin level in patients with lupus nephritis and IgA nephropathy.

Interest of Serum Periostin Dosage in Patients With Bone Fibrous Dysplasia

Fibrous dysplasia is a rare bone disease which can cause pain and fractures. It has been shown that periostin is over expressed in fibrous component in patients bones ; but periostin has n...

Biomarkers of Intestinal Mucosal Healing in Crohn's Disease (P08143)

This study will evaluate biomarkers that reflect changes in gut mucosal status during therapy with infliximab and determine whether changes in the levels of the selected biomarkers can be ...

Analysis of the Nasal Mucosal Immune Response in HIV Infection

The principal objective is to define and compare the viral reservoir, mucosal immune responses and the microbiota of different HIV infection stages; viremic, aviremic (under treatment), na...

Medical and Biotech [MESH] Definitions

Drugs designed to treat inflammation of the nasal passages, generally the result of an infection (more often than not the common cold) or an allergy related condition, e.g., hay fever. The inflammation involves swelling of the mucous membrane that lines the nasal passages and results in inordinate mucus production. The primary class of nasal decongestants are vasoconstrictor agents. (From PharmAssist, The Family Guide to Health and Medicine, 1993)

Inflammation of the NASAL MUCOSA, the mucous membrane lining the NASAL CAVITIES.

An autosomal dominant disorder that is manifested by thickened spongiform ORAL MUCOSA with a white opalescent tint. Other MUCOSAL TISSUE may also be involved mucosa found in the VAGINA; RECTUM, and NASAL CAVITY may be similarly involved. This form of LEUKOKERATOSIS can be caused by a mutation in the gene for KERATIN 4 and is not considered a PRENEOPLASTIC CONDITION.

Fluid obtained by irrigation or washout of the nasal cavity and NASAL MUCOSA. The resulting fluid is used in cytologic and immunologic assays of the nasal mucosa such as with the NASAL PROVOCATION TEST in the diagnosis of nasal hypersensitivity.

A major and the second most common isoform of apolipoprotein E. In humans, Apo E4 differs from APOLIPOPROTEIN E3 at only one residue 112 (cysteine is replaced by arginine), and exhibits a lower resistance to denaturation and greater propensity to form folded intermediates. Apo E4 is a risk factor for ALZHEIMER DISEASE and CARDIOVASCULAR DISEASES.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Surgical treatments
Surgery is a technology consisting of a physical intervention on tissues. All forms of surgery are considered invasive procedures; so-called "noninvasive surgery" usually refers to an excision that does not penetrate the structure being exci...


Searches Linking to this Article