Analysis of the frequencies and functions of CD4CD25CD127, CD4HLA-G, and CD8HLA-G regulatory T cells in pre-eclampsia.

08:00 EDT 21st June 2019 | BioPortfolio

Summary of "Analysis of the frequencies and functions of CD4CD25CD127, CD4HLA-G, and CD8HLA-G regulatory T cells in pre-eclampsia."

Most of the investigations on regulatory T cells (Treg) have focused on CD4CD25Foxp3 Treg cells. Although new subsets of these cells such as CD4CD25CD127, CD4HLA-G, and CD8HLA-G Treg cells have been introduced, documents regarding these populations are limited or controversial in case of pregnancy and pre-eclampsia (PE). Here, we investigated the frequencies of the three aforementioned Treg cell subsets in the peripheral blood of non-pregnant (n = 15), healthy pregnant, and preeclamptic women (n = 17 in each group) using flow cytometry. We also assessed the ability of the isolated CD4CD25CD127 and CD4HLA-G Treg cells to suppress responder T cells proliferation and cytokine secretion using CFSE dye dilution and ELISA technique. Our results showed that the frequency of CD4CD25CD127 Treg cells was significantly lower in preeclamptic women (p =  0.001). Also, this subset negatively correlated with both systolic (R= - 0.401, p =  0.004) and diastolic (R= - 0.541, p =  0.001) blood pressures. Regarding CD4HLA-G and CD8HLA-G Treg cells, the mean percentages of these cells were significantly higher in the context of normal pregnancy (p <  0.01). Finally, our results in the functional assay experiments did not show statistically significant differences between groups (p ≥  0.05), but they reveal a shift toward the lower suppressive capacity of CD4CD25CD127 and CD4HLA-G Treg cells in preeclamptic patients which might be clinically important. In conclusion, a significant decrease in the frequency of Treg cell subsets and also a shift toward the lower suppressive capacity of these cells in preeclamptic patients may lead to immunological maladaptation in the context of PE.


Journal Details

This article was published in the following journal.

Name: Journal of reproductive immunology
ISSN: 1872-7603
Pages: 43-51


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