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Expansins are cell wall-loosening proteins found in all land plants and many microbial species. Despite homologous structures, bacterial expansins have much weaker cellulose binding and wall-loosening activity than plant expansins. We hypothesized stronger cellulose binding would result in greater wall-loosening activity and used in vitro evolution of Bacillus subtilis BsEXLX1 to test this hypothesis. Mutants with stronger binding generally had greater wall-loosening activity, but the relationship was nonlinear and plateaued at ~40% higher than wild type. Mutant E191K exhibited stronger cellulose binding but failed to induce creep, evidently due to protein mistargeting. These results reveal the complexity of interactions between plant cell walls and wall-modifying proteins, an important consideration when engineering proteins for applications in biofuel production and plant pathogen resistance. This article is protected by copyright. All rights reserved.
This article was published in the following journal.
Name: FEBS letters
In this study, bacterial cellulose doped with phosphorus and copper via freeze-drying and high-temperature pyrolysis was used to prepare MFC cathode catalysts. After a series of characterization, the ...
Bacterial cellulose (BC), a valuable biopolymer is gaining importance over the past few decades due to its remarkable properties and applications. In this study, crude distillery effluent having a hig...
Bacterial cellulose aerogels were chosen as the substrate for supporting and dispersing metal nanoparticles (Cu and Ni). During the catalyst preparation, we found that the swelling-induced adsorption ...
A new sustainable synthetic method for cellulose acetate was developed by a combination of I-catalyzed solid-liquid acetylation of cellulose and a milling process reducing the crystallinity of cellulo...
CuO was successfully prepared on bacterial cellulose paper as a nanocomposite using the forced hydrolysis technique. The composite paper presented outstanding photocatalytic and antibacterial properti...
This study is conducted to evaluate the performance of a rapid, point-of-care in vitro diagnostic device (Sinu-Test™) for the qualitative detection of the three most common pathogens res...
This is a Phase 3, multi-center, randomized, placebo-controlled trial to determine the effectiveness and safety of the 6% cellulose sulfate (CS) vaginal gel for the prevention of HIV infec...
Observational: studies in human beings in which biomedical and/or health outcomes are assessed in pre-defined groups of individuals. Subjects in the study may receive diagnostic, therapeut...
The purpose of this study is to determine the in vitro antibiotic susceptibility patterns of conjunctival bacterial flora isolated preoperatively from patients undergoing refractive surger...
In post-dilution haemodiafiltration only synthetic membranes have been used to date. The allergy problems described with these membranes require the development of other membranes capable...
The techniques used to produce molecules exhibiting properties that conform to the demands of the experimenter. These techniques combine methods of generating structural changes with methods of selection. They are also used to examine proposed mechanisms of evolution under in vitro selection conditions.
An isothermal in-vitro nucleotide amplification process. The process involves the concomitant action of a RNA-DIRECTED DNA POLYMERASE, a ribonuclease (RIBONUCLEASES), and DNA-DIRECTED RNA POLYMERASES to synthesize large quantities of sequence-specific RNA and DNA molecules.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
A type of ion exchange chromatography using diethylaminoethyl cellulose (DEAE-CELLULOSE) as a positively charged resin. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The interdisciplinary science that studies evolutionary biology, including the origin and evolution of the major elements required for life, their processing in the interstellar medium and in protostellar systems. This field also includes the study of chemical evolution and the subsequent interactions between evolving biota and planetary evolution as well as the field of biology that deals with the study of extraterrestrial life.
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...