Mechanical stretch enhances sex steroidogenesis in CC Skeletal Muscle Cells.

08:00 EDT 3rd July 2019 | BioPortfolio

Summary of "Mechanical stretch enhances sex steroidogenesis in CC Skeletal Muscle Cells."

Skeletal muscle contains estrogens and estrogen synthesis-related enzymes. However, it has not been reported whether myoblasts cultured in vitro also express these enzymes. The purpose of the current study was to address these issues and to explore the effects of mechanical stretch on the enzyme system. The in vitro cultured CC mouse myoblasts were divided into the control, stretch, testosterone and stretch plus testosterone groups. Cells in the stretch and stretch plus testosterone groups were mechanically stretched with the Flexercell cell stress loading device at an amplitude of 10% and in a frequency of 0.5 Hz for 8 hours. Cells in the testosterone and stretch plus testosterone groups were incubated with 100nM testosterone for 24 hours before distraction. Following the treatments, cell proliferation and estradiol levels, as well as the expressions of 17β-hydroxysteroid (17β-HSD), 3β-hydroxysteroid (3β-HSD) and aromatase were analyzed. Compared to the control, the cell proliferation in all experimental groups increased significantly, the estradiol levels in the mechanically stretched groups were significantly higher, and, moreover, the estradiol levels were positively correlated with the cell proliferation (r = 0.615, p < 0.01). Additionally, analyses of aromatase protein and mRNA showed that, compared to the control, their levels were significantly increased upon stretching and testosterone exposure. Similarly, the protein and mRNA levels of both 3β-HSD and 17β-HSD in the stretched cells differed significantly from the control. In the presence of aromatase and 5α-reductase inhibitors, the protein and mRNA levels of these enzymes altered significantly compared to the control. Conclusions: Steroid synthases were detected in the CC myoblasts cultured in vitro, the synthesized estrogen was closely related to the cell proliferation, and mechanical stretch was the external factor that affected the expression of the estrogen synthesis-related enzymes.


Journal Details

This article was published in the following journal.

Name: Steroids
ISSN: 1878-5867
Pages: 108434


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Medical and Biotech [MESH] Definitions

Skeletal muscle structures that function as the mechanoreceptors responsible for the stretch or myotactic reflex (REFLEX, STRETCH). They are composed of a bundle of encapsulated SKELETAL MUSCLE FIBERS, i.e., the intrafusal fibers (nuclear bag1 fibers, nuclear bag2 fibers, and nuclear chain fibers) innervated by sensory neurons.

Mature contractile cells, commonly known as myocytes, that form one of three kinds of muscle. The three types of muscle cells are skeletal (MUSCLE FIBERS, SKELETAL), cardiac (MYOCYTES, CARDIAC), and smooth (MYOCYTES, SMOOTH MUSCLE). They are derived from embryonic (precursor) muscle cells called MYOBLASTS.

The state of activity or tension of a muscle beyond that related to its physical properties, that is, its active resistance to stretch. In skeletal muscle, tonus is dependent upon efferent innervation. (Stedman, 25th ed)

Autologous skeletal muscle that is wrapped around the heart and electrically stimulated in order to provide mechanical heart assistance. The latissimus dorsi muscle is commonly used to form this ventricle that functions to independently augment cardiac performance by pumping in series with the heart.

Large, multinucleate single cells, either cylindrical or prismatic in shape, that form the basic unit of SKELETAL MUSCLE. They consist of MYOFIBRILS enclosed within and attached to the SARCOLEMMA. They are derived from the fusion of skeletal myoblasts (MYOBLASTS, SKELETAL) into a syncytium, followed by differentiation.

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