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Single-Cell Signature Explorer for comprehensive visualization of single cell signatures across scRNA-seq datasets.

08:00 EDT 11th July 2019 | BioPortfolio

Summary of "Single-Cell Signature Explorer for comprehensive visualization of single cell signatures across scRNA-seq datasets."

The momentum of scRNA-seq datasets prompts for simple and powerful tools exploring their meaningful signatures. Here we present Single-Cell_Signature_Explorer (https://sites.google.com/site/fredsoftwares/products/single-cell-signature-explorer), the first method for qualitative and high-throughput scoring of any gene set-based signature at the single cell level and its visualization using t-SNE or UMAP. By scanning datasets for single or combined signatures, it rapidly maps any multi-gene feature, exemplified here with signatures of cell lineages, biological hallmarks and metabolic pathways in large scRNAseq datasets of human PBMC, melanoma, lung cancer and adult testis.

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Name: Nucleic acids research
ISSN: 1362-4962
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Medical and Biotech [MESH] Definitions

A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.

The movement of CYTOPLASM within a CELL. It serves as an internal transport system for moving essential substances throughout the cell, and in single-celled organisms, such as the AMOEBA, it is responsible for the movement (CELL MOVEMENT) of the entire cell.

Assaying the products of or monitoring various biochemical processes and reactions in an individual cell.

A genus of RED ALGAE in the family Porphyridiaceae. A single stellate CHLOROPLAST is present in each cell which has no CELL WALL.

Mapping of the full chromosome set of the nucleus of a cell. The chromosome characteristics of an individual or a cell line are usually presented as a systematized array of metaphase chromosomes from a photomicrograph of a single cell nucleus arranged in pairs in descending order of size and according to the position of the centromere. (From Stedman, 25th ed)

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