Topics

Dynamic paraspeckle component localization during spermatogenesis.

08:00 EDT 1st July 2019 | BioPortfolio

Summary of "Dynamic paraspeckle component localization during spermatogenesis."

Expression profiles and subcellular localisations of core Drosophila behaviour/human splicing (DBHS) proteins (PSPC1, SFPQ and NONO) and NEAT1, a long non-coding RNA (lncRNA), are investigated in developing and adult mouse testes. Core DBHS proteins are markers for the distinct subnuclear domain termed paraspeckles, while a long NEAT1 isoform scaffold facilitates paraspeckle nucleation. Paraspeckles contain many proteins (>40) and are broadly involved in RNA metabolism, including transcriptional regulation by protein sequestration, nuclear retention of A-to-I edited RNA transcripts to regulate translation, and promoting survival during cellular stress. Immunohistochemistry reveals cell-specific profiles for core DBHS paraspeckle protein expression, indicating their functional diversity. PSPC1 is an androgen receptor co-activator, and it is detected in differentiating Sertoli cell nuclei from day 15 onwards, as they develop androgen-responsiveness. PSPC1 is nuclear in the most mature male germ cell type present at each age, from foetal to adult life. In adult mouse testes, PSPC1 and SFPQ are present in Sertoli cells, spermatocytes and round spermatids, while the NEAT1 lncRNA appears in the punctate nuclear foci delineating paraspeckles only within Leydig cells. Identification of NEAT1 in the cytoplasm of spermatogonia and spermatocytes must reflects non-paraspeckle-related functions. NONO was absent from germ cells but nuclear in Sertoli cells. Reciprocal nuclear profiles of PSPC1 and γ H2AX in spermatogenic cells suggest these each perform developmentally-regulated roles in stress responses. These findings demonstrate paraspeckles and paraspeckle-related proteins contribute to diverse functions during testis development and spermatogenesis.

Affiliation

Journal Details

This article was published in the following journal.

Name: Reproduction (Cambridge, England)
ISSN: 1741-7899
Pages:

Links

DeepDyve research library

PubMed Articles [5028 Associated PubMed Articles listed on BioPortfolio]

Preoperative localization of breast lesions: Current techniques.

Image-guided preoperative localization of breast lesions is a common procedure. This article describes several commercially available localization options-wire localization, radioactive seed localizat...

CFTR mutation compromises spermatogenesis by enhancing miR-15b maturation and suppressing its regulatory target CDC25A.

MicroRNAs (miRNAs) have recently been shown to be important for spermatogenesis; both DROSHA and Dicer1 KO mice exhibit infertility due to abnormal miRNA expression. However, the roles of individual m...

Effects of estrogen on spermatogenesis in transgender women.

To characterize spermatogenesis in the estrogenized transgender patient.

Progesterone Receptor Membrane Component 1 and 2 regulate granulosa cell mitosis and survival through a NFΚB-dependent mechanism.

Progesterone Receptor Membrane Component 1 (PGRMC1) interacts with PGRMC2 and disrupting this interaction in spontaneously immortalized granulosa cells (SIGCS) leads to an inappropriate entry into the...

Structure Dominates Localization of Tracers within Aging Nanoparticle Glasses.

We investigate the transport and localization of tracer probes in a glassy matrix as a function of relative size using dynamic x-ray scattering experiments and molecular dynamics simulations. The quie...

Clinical Trials [1301 Associated Clinical Trials listed on BioPortfolio]

The Happy Older Latinos Are Active (HOLA) Health Promotion Study in HIV-Infected Latino Men

The purpose of this study is to look at the best ways to prevent chronic diseases like diabetes, high blood pressure, and obesity in midlife and older Latino adults living with HIV. The in...

A Study Comparing the Efficacy and Safety of IDP-120 Gel in the Treatment of Acne Vulgaris

The primary objective of this study is to compare the efficacy, safety, and tolerability of IDP-120 Gel to IDP-120 Component A, IDP-120 Component B, and IDP-120 Vehicle Gel in subjects wit...

Evaluating Methods to Increase HIV Testing, Access to HIV Care, and HIV Prevention Strategies

This is a five-part study that will take place in the Bronx, NY, and Washington, D.C. The different components of the study will focus on increasing the number of people being tested for H...

Preoperative Stereoscopic Localization Versus Methylene Blue Localization in GGO Wedge Resection

This study evaluates the feasibility,accuracy and safety of Preoperative Stereoscopic Localization Versus Methylene Blue Localization in GGO Wedge Resection. In the first stage,partici...

Preventing Anxiety and Depression in Older Hispanics

The purpose of this study is to look at the best ways to prevent anxiety and depression in older Latino adults who are at risk for developing anxiety and depression. Participants will be r...

Medical and Biotech [MESH] Definitions

A minichromosome maintenance protein that is a key component of the six member MCM protein complex. It contains a NUCLEAR LOCALIZATION SIGNAL which may provide targeting of the protein complex and an extended N-terminus which is rich in SERINE residues.

A minichromosome maintenance protein that is a key component of the six member MCM protein complex. It contains a NUCLEAR LOCALIZATION SIGNAL, which provide targeting of the protein complex. In addition, acetylation of this protein may play a role in regulating of DNA replication and cell cycle progression.

A minichromosome maintenance protein that is a key component of the six member MCM protein complex. It is also found in tightly-bound trimeric complex with MINICHROMOSOME MAINTENANCE COMPLEX COMPONENT 6 and MINICHROMOSOME MAINTENANCE COMPLEX COMPONENT 7.

A minichromosome maintenance protein that is a key component of the six member MCM protein complex. It is also found in tightly-bound trimeric complex with MINICHROMOSOME MAINTENANCE COMPLEX COMPONENT 4 and MINICHROMOSOME MAINTENANCE COMPLEX COMPONENT 7.

A minichromosome maintenance protein that is a key component of the six member MCM protein complex. It is also found in tightly-bound trimeric complex with MINICHROMOSOME MAINTENANCE COMPLEX COMPONENT 4 and MINICHROMOSOME MAINTENANCE COMPLEX COMPONENT 6.

Quick Search


DeepDyve research library

Relevant Topic

Stress
Stress is caused by your perception of situations around you and then the reaction of your body to them. The automatic stress response to unexpected events is known as 'fight or flight'. Discovered by Walter Cannon in 1932, it is the release of h...


Searches Linking to this Article