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Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids such as blood is important in biomedical research and clinical applications. A surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) is developed for the quantitative analysis of IL-6. The Raman reporter 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) on gold nano shell with a silica core was employed as the SERS tags. They are shown to perform much better than colloidal gold in LF strips. The IL-6 protein can be detected by this method with very low detection limits by monitoring the intensity of the characteristic Raman peak of the IL-6-conjugated SERS tags at 1332 cm⁻. Under optimized conditions, the assay works in the 1 pg/mL to 1 μg/mL IL-6 concentration range, and the detection limit is as low as 1 pg/mL in PBS, 5 pg/mL in unprocessed whole blood. This is lower by a factor of 3 compared to colorimetric or fluorimetric methods. The performance of SERS LFA was demonstrated by detection of IL-6 in unprocessed whole blood with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).
This article was published in the following journal.
Name: Biosensors & bioelectronics
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A quantitative prediction of the biological, ecotoxicological or pharmaceutical activity of a molecule. It is based upon structure and activity information gathered from a series of similar compounds.
Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).
Locations, on the GENOME, of GENES or other genetic elements that encode or control the expression of a quantitative trait (QUANTITATIVE TRAIT, HERITABLE).
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
A characteristic showing quantitative inheritance such as SKIN PIGMENTATION in humans. (From A Dictionary of Genetics, 4th ed)
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