A SERS-based lateral flow assay biosensor for quantitative and ultrasensitive detection of interleukin-6 in unprocessed whole blood.

08:00 EDT 19th June 2019 | BioPortfolio

Summary of "A SERS-based lateral flow assay biosensor for quantitative and ultrasensitive detection of interleukin-6 in unprocessed whole blood."

Detection of a very low amount of cytokines such as interleukin-6 (IL-6) in clinical fluids such as blood is important in biomedical research and clinical applications. A surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) is developed for the quantitative analysis of IL-6. The Raman reporter 5,5'-dithiobis-2-nitrobenzoic acid (DTNB) on gold nano shell with a silica core was employed as the SERS tags. They are shown to perform much better than colloidal gold in LF strips. The IL-6 protein can be detected by this method with very low detection limits by monitoring the intensity of the characteristic Raman peak of the IL-6-conjugated SERS tags at 1332 cm⁻. Under optimized conditions, the assay works in the 1 pg/mL to 1 μg/mL IL-6 concentration range, and the detection limit is as low as 1 pg/mL in PBS, 5 pg/mL in unprocessed whole blood. This is lower by a factor of 3 compared to colorimetric or fluorimetric methods. The performance of SERS LFA was demonstrated by detection of IL-6 in unprocessed whole blood with comparable performance of the conventional enzyme-linked immunosorbent assay (ELISA).


Journal Details

This article was published in the following journal.

Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages: 111432


DeepDyve research library

PubMed Articles [24140 Associated PubMed Articles listed on BioPortfolio]

An enzymatic paper-based biosensor for ultrasensitive detection of DNA.

Point-of-care Nucleic acid testing (POCNAT) has become an attractive technique for DNA identification in resource-limited settings, offering a rapid system for urgent clinical applications. In this st...

A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin.

For modern approaches in precision medicine, fast and easy-to-use point-of-care diagnostics (POCs) are essential. Digoxin was chosen as an example of a drug requiring close monitoring. Digoxin is a ca...

Hierarchic Interfacial Nanocube Assembly for Sensitive, Selective, and Quantitative DNA Detection with Surface-Enhanced Raman Scattering.

Surface-enhanced Raman scattering (SERS)-based sensing is promising in that it has potential to allow for highly sensitive, selective, and multiplexed detection and imaging. However, the controlled as...

Surface plasmon resonance biosensor for the ultrasensitive detection of bisphenol A.

Bisphenol A (BpA) is a chemical that is extensively used in common plastic products, such as food and drink containers. It can leach from the plastics and penetrate into the human body, where it acts ...

Ultrafast, universal and visual screening of dual genetically modified elements based on dual super PCR and a lateral flow biosensor.

In this study, a cascade screening system has been developed combining Dual Super Polymerase Chain Reaction (DSPCR) with the universal Lateral Flow Biosensor (LFB) for the ultrafast, universal and vis...

Clinical Trials [7951 Associated Clinical Trials listed on BioPortfolio]

LAMP Assay for the Diagnosis of Visceral Leishmaniasis

This study will evaluate the of the loop-mediated amplification assay (LAMP) as a diagnostic as well as a Test-of-Cure (ToC) for visceral leishmaniasis (VL) in an endemic area in Ethiopia....

OstiSense Biosensor in Bruxism Reduction - A Clinical Study

The overall objective of the clinical studies with the OstiSense biosensor tool will be to evaluate whether the use of the OstiSense biosensor tool significantly reduces the number of slee...

Trial of Blood Flow Restriction Plus Physical Therapy vs. Physical Therapy Alone for Lateral Epicondylitis

Open label randomized controlled trial comparing evidenced based physical/occupational therapy vs. physical/occupational therapy including blood flow restriction tourniquet in the treatmen...

Early Assesment of QFR in STEMI-II

The study intends to provide new data on whether the noval method using quantitative flow ratio could assess microvascular dysfunction based on the previous study EARLY-MYO-QFR-I.

Diagnostic Accuracy of On-line Quantitative Flow Ratio

Quantitative Flow Ratio (QFR) is a novel method for evaluating the functional significance of coronary stenosis. QFR is assessed by calculation of the pressure in the vessel based on two a...

Medical and Biotech [MESH] Definitions

A quantitative prediction of the biological, ecotoxicological or pharmaceutical activity of a molecule. It is based upon structure and activity information gathered from a series of similar compounds.

Quantitative determination of receptor (binding) proteins in body fluids or tissue using radioactively labeled binding reagents (e.g., antibodies, intracellular receptors, plasma binders).

Locations, on the GENOME, of GENES or other genetic elements that encode or control the expression of a quantitative trait (QUANTITATIVE TRAIT, HERITABLE).

Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.

A characteristic showing quantitative inheritance such as SKIN PIGMENTATION in humans. (From A Dictionary of Genetics, 4th ed)

Quick Search

DeepDyve research library

Relevant Topics

An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article