Topics

Rhamnogalacturonan I galactosyltransferase: Detection of enzyme activity and its hyperactivation.

08:00 EDT 4th July 2019 | BioPortfolio

Summary of "Rhamnogalacturonan I galactosyltransferase: Detection of enzyme activity and its hyperactivation."

Rhamnogalacturonan I (RG-I), one of the pectic components of the plant cell wall, is composed of a backbone of repeating disaccharide units of rhamnose and galacturonic acid, and side chains, such as galactans, arabinans, and arabinogalactans. The activity of RG-I galactosyltransferase, which transfers galactosyl residues to rhamnosyl residues in the RG-I backbone, has not been detected until now. Here, we detected galactosyltransferase activity in azuki bean epicotyls using fluorogenic RG-I oligosaccharide acceptors. This enzyme prefers oligosaccharides with a degree of polymerization more than 9. The enzyme activity was detected in the Golgi apparatus, which is the site of pectin synthesis. In vitro hyperactivation of this enzyme was also observed. Moreover, enzyme activity was increased up to 40-fold in the presence of cationic surfactants or polyelectrolytes.

Affiliation

Journal Details

This article was published in the following journal.

Name: Plant physiology and biochemistry : PPB
ISSN: 1873-2690
Pages: 173-178

Links

DeepDyve research library

PubMed Articles [17248 Associated PubMed Articles listed on BioPortfolio]

A DNA functionalized porphyrinic metal-organic framework as a peroxidase mimicking catalyst for amperometric determination of the activity of T4 polynucleotide kinase.

An electrochemical method is described for the sensitive detection of the activity of the enzyme T4 polynucleotide kinase (PNK) by using a DNA functionalized porphyrinic metal-organic framework (L/(Fe...

Hollow copper sulfide nanocubes as multifunctional nanozymes for colorimetric detection of dopamine and electrochemical detection of glucose.

Nanozymes have fascinated increasing attention in the field of artificial enzyme. Designing an ideal nanozyme usually requires a synergic advantage of reasonable nanostructures and large specific surf...

A novel metalloprotease from banana peel and its biochemical characterization.

Protein cleaving enzymes, called proteases are one of the most commercially used enzymes possessing extensive applications in various industries. The present study was focused on screening, extraction...

Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein.

Ficin extract has been aminated using ethylenediamine and carbodiimide to transform all exposed carboxylic groups into amino groups, retaining around 80% of activity versus benzoyl-d,l-arginine p-nitr...

Metabolism of polysaccharides in dynamic middle lamellae during cotton fibre development.

Evidence is presented that cotton fibre adhesion and middle lamella formation are preceded by cutin dilution and accompanied by rhamnogalacturonan-I metabolism. Cotton fibres are single cell structure...

Clinical Trials [5922 Associated Clinical Trials listed on BioPortfolio]

Consumption of Dairy Yogurt Enhanced Immune Function

To investigate the impact of consumption of dairy yogurt with rhamnogalacturonan (RG) in Korean citrus Hallabong peel polysaccharide on Natural Killer (NK) cell activity and circulating cy...

Evaluation of the Entresto Effect on Sympathic Nervous System in Patient With Heart Failure

The hyperactivation of the sympathetic nervous system is a feature of the heart failure and the determinants of disease progression and risk of sudden cardiac death. This research project ...

Rapid Screening Phenotype Test To Evaluate CYP 2C19 Enzyme Activity Using Stable Isotope [13C]Pantoprazole

The purpose of this study is to determine the function of an enzyme that breaks down drugs and helps the removal of drugs from your body. This enzyme is called cytochrome P450 2C19 and is...

Prolidase Enzyme Activity in Stroke Patients

Stroke is a major cerebrovascular disease that causes significant burdens for human health and life, including high morbidity, mortality, and disability. Prolidase enzyme activity was foun...

Lipoprotein Lipase Enzyme Activity Assay Validation and Clinical Assessment

This study plans to learn more about measuring Lipoprotein lipase (LPL) activity in humans. LPL is an enzyme in the breakdown of certain types of fats into smaller parts. Lipoprotein lipas...

Medical and Biotech [MESH] Definitions

Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.

An enzyme that catalyzes the conversion of UDP-galactose and N-acylsphingosine to D-galactosylceramide and UDP.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

An enzyme that catalyzes the transfer of galactose from UDP-galactose to a specific glycoprotein receptor, 2-acetamido-2-deoxy-D-glucosyl-glycopeptide, during glycopeptide synthesis. EC 2.4.1.38.

Quick Search


DeepDyve research library

Relevant Topic

Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...


Searches Linking to this Article