Protein recognition by polydopamine-based molecularly imprinted hollow spheres.

08:00 EDT 5th July 2019 | BioPortfolio

Summary of "Protein recognition by polydopamine-based molecularly imprinted hollow spheres."

A facile method to prepare hollow molecularly imprinted polymers (HMIPs) for specific recognition of horseradish peroxidase (HRP) from biological samples was proposed in this paper. The HMIPs was prepared using silica nanoparticles as the sacrificial matrix and dopamine as functional monomer. The thickness of polydopamine shells can be easily modulated by tuning the mass ratio of silica matrix and dopamine. The polymerization conditions and recognition behaviors of the HMIPs were investigated systematically. The results suggested that the hollow structure endowed the HMIPs with fast adsorption kinetics of 25 min, high binding capacity of 172.1 mg/g, and reusability of no less than four adsorption-regeneration cycles without apparent deterioration. Meanwhile, excellent binding specificity towards HRP was presented in the selectivity studies. Moreover, enriching of HRP from human serum sample by the obtained HMIPs was conducted. The HMIPs displayed satisfactory binding specificity to HRP, in spite of the complex composition of the human serum.


Journal Details

This article was published in the following journal.

Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages: 111492


DeepDyve research library

PubMed Articles [24404 Associated PubMed Articles listed on BioPortfolio]

A novel electrochemical sensor based on molecularly imprinted polymer modified hollow N, S-MoC/C spheres for highly sensitive and selective carbendazim determination.

A novel electrochemical sensor based on nitrogen and sulfur doped hollow MoC/C spheres (N, S-MoC) and molecularly imprinted polymer (MIP) was proposed for carbendazim (CBD) determination. The N, S-MoC...

Multi-templates based molecularly imprinted sodium alginate/MnO for simultaneous enantiorecognition of lysine, alanine and cysteine isomers.

Sodium alginate (SA) was electrodeposited onto the surface of a glassy carbon electrode (GCE), and then l-lysine (l-Lys), l-alanine (l-Ala) and l-cysteine (l-Cys) were simultaneously introduced to the...

Modification of nanocrystalline TiO coatings with molecularly imprinted TiO for uric acid recognition.

Combining the surface modification and molecular imprinting technique, a novel piezoelectric sensing platform with excellent molecular recognition capability was established for the detection of uric ...

CoS nanosheets wrapping on bowl-like hollow carbon spheres with enhanced compact density for sodium-ion batteries.

Transition metal sulfides are long suffered from poor conductivity and large volume change when valued as anode material for sodium-ion batteries. Hollow carbon nanohybrids prove to be the highly effe...

Synthesis of hollow MoC/carbon spheres, and their application to simultaneous electrochemical detection of hydroquinone, catechol, and resorcinol.

Hollow molybdenum-dopamine spheres were synthesized and thermally annealed to form hollow MoC/C spheres. The morphology, composition and electrochemical behavior of spheres were characterized. A glass...

Clinical Trials [5773 Associated Clinical Trials listed on BioPortfolio]

CIRSE Registry for SIR-Spheres in France (CIRT-FR)

Selective Internal Radiation Therapy (SIRT), also known as radioembolisation, is a minimal invasive, endovascular treatment for primary and secondary liver tumours. In France, SIR-Spheres ...

SIR-Spheres® for the Treatment of Unresectable Hepatocellular Carcinoma

The main purpose of this study is to determine the safety and toxicity of treatment with SIR-Spheres® in patients with unresectable primary liver cancer or hepatocellular carcinoma (HCC)....

Feasibility of Single Session In-Room Yttrium-90 Radioembolization Diagnostic Angiography and Treatment

The goal of this clinical research study is to learn about the safety of giving SIR-Spheres treatment to patients with liver cancer in the same session rather than over 2 sessions, which i...

Study of Capecitabine in Combination With SIR-Spheres in Patients With Advanced Cancer

This trial is testing the safety of combining the oral chemotherapy drug capecitabine with radio-labeled microspheres injected directly into the liver.

Identification of New Inborn Errors of Immunity

Analysis of DNA samples of patients with molecularly undetermined PID by whole exome/genome sequencing. Transcriptome analysis of patients with molecularly undetermined PID.

Medical and Biotech [MESH] Definitions

A methodology for chemically synthesizing polymer molds of specific molecules or recognition sites of specific molecules. Applications for molecularly imprinted polymers (MIPs) include separations, assays and biosensors, and catalysis.

The origin recognition complex is a multi-subunit DNA-binding protein that initiates DNA REPLICATION in eukaryotes.

A family of RNA-BINDING PROTEINS that contain an RNA RECOGNITION MOTIF and two ribonucleoprotein (RNP) domains which bind RNA, in addition to other domains that allow for high affinity binding, sequence specificity, and protein interactions. Examples of RNA recognition motif proteins include HETEROGENEOUS NUCLEAR RIBONUCLEARPROTEINS (hnRNP) and EMBRYONIC LETHAL ABNORMAL-VISION (ELAV) proteins.

A method of differentiating individuals based on the analysis of qualitative or quantitative biological traits or patterns. This process which has applications in forensics and identity theft prevention includes DNA profiles or DNA fingerprints, hand fingerprints, automated facial recognition, iris scan, hand geometry, retinal scan, vascular patterns, automated voice pattern recognition, and ultrasound of fingers.

A small maf protein that forms dimers with NRF1 protein; NRF2 PROTEIN; and P45 NF-E2 PROTEIN. MafF complexes bind Maf recognition elements to regulate tissue-specific GENETIC TRANSCRIPTION.

Quick Search

DeepDyve research library

Relevant Topics

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Searches Linking to this Article