Embedding inulin fructotransferase from Arthrobacter aurescens into novel curdlan-based mesoporous silica microspheres for efficient production of Difructose Anhydride III.

08:00 EDT 4th July 2019 | BioPortfolio

Summary of "Embedding inulin fructotransferase from Arthrobacter aurescens into novel curdlan-based mesoporous silica microspheres for efficient production of Difructose Anhydride III."

A novel strategy was used to produce inulin fructotransferase from Arthrobacter aurescens (Aa-IFTase) embedded in curdlan-based mesoporous silica microspheres (CMSiM-Aa-IFTase). The CMSiM-Aa-IFTase was constructed by co-entrapping cross-linked Aa-IFTase aggregates and curdlan into biomemitic silica, and the curdlan was subsequently removed by digestion with endo-β-1,3-glucanase. During this process, the curdlan served as an agent to introduce pores in silica microspheres. The resulting CMSiM-Aa-IFTase showed higher stability and activity than free Aa-IFTase and mCLEAs-Aa-IFTase (modified cross-linked enzyme aggregates with Aa-IFTase). Furthermore, the CMSiM-Aa-IFTase displayed good reusability and excellent storage stability. The excellent catalytic performances were due to the combinational structure from the cross-linked enzyme aggregates and hard shell of mesoporous silica microspheres, which might decrease the negative interaction between support and enzyme, and improve the mechanical properties. The CMSiM-Aa-IFTase was applicable for efficient production of Difructose Anhydride III (DFA III), and this approach should be highly valuable for preparing various mesoporous composites for catalysis.


Journal Details

This article was published in the following journal.

Name: Food chemistry
ISSN: 1873-7072
Pages: 125128


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