Fabrication and characterization of extracellular matrix scaffolds obtained from adipose-derived stem cells.

08:00 EDT 9th July 2019 | BioPortfolio

Summary of "Fabrication and characterization of extracellular matrix scaffolds obtained from adipose-derived stem cells."

Chronic non-healing wounds are detrimental for the quality of life of the affected individuals and represent a major burden for the health care systems. Adipose-derived stem cells (ASCs) are being investigated for the development of novel treatments of chronic wounds, as they have shown several positive effects on wound healing. While these effects appear to be mediated by the release of soluble factors, it is has also become apparent that the extracellular matrix (ECM) deposited by ASCs is essential in several phases of the wound healing process. In this work, we describe an approach to produce ECM scaffolds derived from ASCs in culture. Upon growth of ASCs into an overconfluent cell layer, a detergent-based cell extraction approach is applied to remove the cellular components. The extraction is followed by an enzymatic treatment to remove the residual DNA. The resultant cell-derived scaffolds are depleted of cellular components, display low DNA remnant, and retain the native fibrillar organization of the ECM. Analysis of the molecular composition of the ECM scaffolds revealed that they are composed of collagens type I and III, and fibronectin. The decellularized scaffolds represent a substrate that supports adhesion and proliferation of primary human fibroblasts and dermal microvascular endothelial cells, indicating their potential as platforms for wound healing studies.


Journal Details

This article was published in the following journal.

Name: Methods (San Diego, Calif.)
ISSN: 1095-9130


DeepDyve research library

PubMed Articles [15156 Associated PubMed Articles listed on BioPortfolio]

Preparation and Characterization of Tissue Surrogates Rich in Extracellular Matrix Using the Principles of Macromolecular Crowding.

Tissue engineering by self-assembly allows for the fabrication of living tissue surrogates by taking advantage of the cell's inherent ability to produce and deposit tissue-specific extracellular matri...

Keratin Scaffolds with Human Adipose Stem Cells: Physical and Biological Effects toward Wound Healing.

Keratin, a natural biomaterial derived from wool or human hair, has the intrinsic ability to interact with different types of cells and the potential to serve as a controllable extracellular matrix wh...

Tissue Engineered Soft Tissue Reconstruction Using Non-invasive Mechanical Preconditioning and a Shelf-ready Allograft Adipose Matrix.

Adipose tissue defects leading to severe functional (disability) and morphological (disfigurement) morbidity are often treated in plastic surgery with fat grafting, which can be limited by resorption,...

Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy.

Scanning electron microscopy is a useful tool for high-resolution morphological characterization of the extracellular matrix. In certain tissues and surfaces, imaging of the extracellular matrix requi...

Fabrication of a nanoparticle-containing 3D porous bone scaffold with proangiogenic and antibacterial properties.

3D porous scaffolds based on agarose and nanocrystalline apatite, two structural components that act as a temporary mineralized extracellular matrix, were prepared by the GELPOR3D method. This shaping...

Clinical Trials [3419 Associated Clinical Trials listed on BioPortfolio]

Pilot Study to Evaluate the Safety and the Efficacy of the Modified Matrix Obtained by the AmeaCell® Device and Intended for the Filling of Wrinkles on the Face.

The purpose of this study is to evaluate the filling using the modified matrix, obtained by the Ameacell® device from autologous adipose tissue and intended for the filling of wrinkles on...

Role of Extracellular Matrix in the Development of Airway Remodeling in Asthma

Asthma is a major noncommunicable chronic inflammatory disorder which is characterized by airway inflammation and related to pathological modifications of the bronchial wall structure so c...

Endoscopic Mucosal Resection and Cellular Matrix

This study is being done to assess the use of scaffold products in promoting constructive healing as determined by the ability of the resection site to lift during follow-up.

Sputum Matrix Metalloproteinases (MMP) mRNA and Montelukast

Matrix metalloproteinases (MMPs) are a group of 24 zinc containing enzymes in man. These enzymes were originally described as cleaving extracellular matrix (ECM) substrates with a predo...

The Role of Estrogen in Adipocyte Remodeling Following Surgical Menopause

The overarching aim of this study is to assess the downstream effects of abrupt estrogen deficiency in women undergoing elective bilateral oophorectomy by studying: 1. the rate of in vi...

Medical and Biotech [MESH] Definitions

A family of extracellular matrix glycoproteins that is structurally similar to LATENT TGF-BETA BINDING PROTEINS, but contain additional TGF-beta binding domains, in addition to unique domains at their N and C-terminals. Fibrillins assemble into 10-12 nm MICROFIBRILS that function in a variety of cell interactions with the EXTRACELLULAR MATRIX and developmental processes such as ELASTIC TISSUE maintenance and assembly, and the targeting of growth factors to the extracellular matrix.

PROTEOGLYCANS-associated proteins that are major components of EXTRACELLULAR MATRIX of various tissues including CARTILAGE; and INTERVERTEBRAL DISC structures. They bind COLLAGEN fibers and contain protein domains that enable oligomer formation and interaction with other extracellular matrix proteins such as CARTILAGE OLIGOMERIC MATRIX PROTEIN.

Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).

A secreted matrix metalloproteinase that is believed to play a role in EXTRACELLULAR MATRIX remodeling and cell fate determination during normal and pathological processes. Matrix metalloproteinase 11 was originally isolated in primary BREAST NEOPLASMS and may be involved in the process of tumorigenesis.

Hexameric extracellular matrix glycoprotein transiently expressed in many developing organs and often re-expressed in tumors. It is present in the central and peripheral nervous systems as well as in smooth muscle and tendons. (From Kreis & Vale, Guidebook to the Extracellular Matrix and Adhesion Proteins, 1993, p93)

Quick Search


DeepDyve research library

Relevant Topics

Stem Cells
Track and monitor developments in stem cell research and commercial development.  Follow the tabs above to read the latest global news, research, clinical trials on stem cells and follow companies active in the stem cell industry.  BioPort...

Complementary and Alternative Medicine
Alternative medicine are whole medical systems that did not fit with conventional medicine as they have completely different philosophies and ideas on the causes of disease, methods of diagnosis and approaches to treatment. Although often overlapping, co...

Wound management
Anything that breaks the skin is a wound because when the skin is broken, there's a risk of germs getting into the body and causing an infection. Follow and track Wound Care News on BioPortfolio: Wound Car...

Searches Linking to this Article