Sub-micron sized cytochrome c particles adsorbing to solid surfaces: A comparison between solution phase and colloidal system.

08:00 EDT 9th July 2019 | BioPortfolio

Summary of "Sub-micron sized cytochrome c particles adsorbing to solid surfaces: A comparison between solution phase and colloidal system."

Present work reports on Cytochrome C (Cyt C) adsorption from solution and as sub-micron sized colloidal particles (pH 7.5) at fluid/solid interface. The colloidal particles from 2 methods ((method 1 freeze-thaw); method 2-Decane/water interface) have been characterized by UV-Visible spectroscopy, Static and Time resolved Fluorescence spectra and Circular dichroic spectroscopy. Morphology and size studies indicate that the colloids are solid and well-packed. Using Quartz crystal microbalance with dissipation, elastic compliance of the protein on quartz surface has been monitored. Properties of the protein from solution and as colloids assembled from method 2 are similar in elastic compliance (65.53 ± 1.2 and 73.5 ± 1.1 GPa respectively) due to polar/non-polar interactions at the solid surface. For particles from method 1, irregular desolvation of water on the particle surface results in higher compliance (104.3 ± 1.3 GPa). Change in work of adhesion from contact angle profiles shows optimal adhesion for colloids from method 1 whereas the protein solution and as colloids from method 2, show subnormal adhesion. The work shows the role of extensive H-bonding with the hydrodynamically coupled water leads to a fluid like system in protein colloids from method 1 whereas those from method 2 behaved more like the pure protein adsorbing from solution.


Journal Details

This article was published in the following journal.

Name: International journal of biological macromolecules
ISSN: 1879-0003


DeepDyve research library

PubMed Articles [9588 Associated PubMed Articles listed on BioPortfolio]

Mechanical contact spectroscopy: characterizing nanoscale adhesive contacts via thermal forces.

Adhesion of micro- and nanoparticles to solid substrates immersed in liquids is a problem of great scientific and technological importance. Yet, quantitative characterization of such nanoscale adhesiv...

Electrooptical determination of the isoelectric point of globular proteins: Cytochrome c adsorbed on montmorillonite nanoplates.

The purpose is to study the capability of the electric light scattering to determine the point of zero charge of native protein macromolecules adsorbed on colloid particles at low ionic strength and w...

Controlled Deposition of Nano- and Micro-Size Particles by Spin Casting.

The deposition of nano and micron size spherical particles on planar solid substrates by hydrodynamic-evaporative spin casting is studied. The particles are dispersed in a volatile liquid, which evapo...

Colloidal stabilizer-assisted polymerization-induced precipitation method toward colloidally stable polyacid particles.

Near-monodispersed, colloidally stable, submicrometer-sized poly(acid phosphoxy ethyl methacrylate) (PAPEMA) latex particles were synthesized by free radical dispersion polymerization using poly(N-vin...

Photo-controlled growth of polymeric submicron-sized particles.

A tripodal coumarin derivative shows complex photoreactivity, changing from intra- to intermolecular photodimerization with increasing concentration. At high concentration, the compound undergoes effi...

Clinical Trials [6268 Associated Clinical Trials listed on BioPortfolio]

Does Extra-Fine Hydrofluoroalkane-Beclomethasone Dipropionate (HFA-BDP) Suppress Small Airways Inflammation in Chronic Obstructive Pulmonary Disease (COPD)?

Chronic obstructive pulmonary disease or 'COPD' is a chronic disease, which means that it cannot be cured, but that inhalers and tablets can be used to control the symptoms. In COPD, the a...

The Impact of Cytochrome P450 Abnormalities in Patients With Delirium.

Objectives: 1. To examine whether patients with delirium have higher prevalence of cytochrome-P450 abnormalities compared to patients without delirium. 2. To examine wheth...

Woodsmoke Particulate + Hypertonic Saline

Deployment of military personnel has been associated with increased respiratory illness likely due, in part, to inhalation of unusual particulate matter (PM), such as from burn pits. Infla...

Bio Metrology of Nonfibrous Mineral Particles in Bronchoalveolar Lavage Fluids

The main purpose of this study is to update the pulmonary retention values of non-fibrous mineral particles in the general population. This study will provide reference values for the int...

Effect of Rosuvastatin Therapy on HDL2 Level

In many large trials, reducing low density lipoprotein (LDL) levels with rosuvastatin decreased the incidence of major cardiovascular events,but little attention to the effects of rosuva...

Medical and Biotech [MESH] Definitions

Comparison of various psychological, sociological, or cultural factors in order to assess the similarities or diversities occurring in two or more different cultures or societies.

Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.

The interactions of particles responsible for their scattering and transformations (decays and reactions). Because of interactions, an isolated particle may decay into other particles. Two particles passing near each other may transform, perhaps into the same particles but with changed momenta (elastic scattering) or into other particles (inelastic scattering). Interactions fall into three groups: strong, electromagnetic, and weak. (From McGraw-Hill Encyclopedia of Science & Technology, 7th ed)

Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.

A protein complex that includes CYTOCHROME B6 and CYTOCHROME F. It is found in the THYLAKOID MEMBRANE and plays an important role in process of PHOTOSYNTHESIS by transferring electrons from PLASTOQUINONE to PLASTOCYANIN or CYTOCHROME C6. The transfer of electrons is coupled to the transport of PROTONS across the membrane.

Quick Search


DeepDyve research library

Relevant Topic

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article