Molecular typing of Streptococcus agalactiae isolates of serotype Ia from tilapia in southern China.

08:00 EDT 12th July 2019 | BioPortfolio

Summary of "Molecular typing of Streptococcus agalactiae isolates of serotype Ia from tilapia in southern China."

Streptococcus agalactiae is an important pathogen of tilapia causing enormous economic losses worldwide. In this study, multi-locus sequence typing indicated that 75 S. agalactiae isolates from tilapia in southern China were of the ST-7, as well as, belonging to serotype Ia confirmed by a multiplex PCR assay. The putative-virulence gene profiles and genetic variation of these strains were determined by three sets of multiplex PCR and multi-virulence locus sequencing typing (MVLST), respectively. Analysis of putative-virulence gene profiles showed that each strain harbored 18 putative-virulence genes but lacked lmb and scpB. Three putative-virulence genes (srr-1, bibA, and fbsA) were further selected for MVLST analysis. Our data showed that the strains had 14 MVLST types (1-14) and clustered in three groups (Groups I-Ⅲ). A period of time between 2013 and 2014 was an important turning point for the differentiation of the putative-virulence genes of S. agalactiae, as type 1 within Groups Ⅱ became the predominant MVLST type. There were significant differences in MVLST types of S. agalactiae isolated from different tilapia farming regions. MVLST assay may improve the discriminatory power and is suitable for understanding the epidemiology of S. agalactiae serotype Ia and screening multivalent vaccine candidate strains.


Journal Details

This article was published in the following journal.

Name: FEMS microbiology letters
ISSN: 1574-6968


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