Two Novel Genomospecies in the Agrobacterium tumefaciens Species Complex Associated with Rose Crown Gall.

08:00 EDT 12th July 2019 | BioPortfolio

Summary of "Two Novel Genomospecies in the Agrobacterium tumefaciens Species Complex Associated with Rose Crown Gall."

In this study, we explored the pathogenicity and phylogenetic position of Agrobacterium spp. strains isolated from crown gall tissues on annual, perennial and ornamental plants in Iran. Of the 43 strains studied, 10 strains were identified as Allorhizobium vitis (formerly Agrobacterium vitis) using the species-specific primer pair PGF/PGR. Thirty-three remaining strains were studied using multilocus sequence analysis of four housekeeping genes (i.e., atpD, gyrB, recA and rpoB), from which seven strains were identified as A. larrymoorei and one strain as A. rubi (Rer), while the remaining 25 strains were scattered within the A. tumefaciens species complex. Two strains were identified as genomospecies 1 (G1), seven strains as A. radiobacter (G4), seven strains as A. deltaense (G7), two strains as A. nepotum (G14), and one strain as "A. viscosum" (G15). The strains Rnr, Rnw and Rew, as well as the two strains OT33 and R13 all isolated from rose, and the strain Ap1 isolated from apple were clustered in three atypical clades within the A. tumefaciens species complex. All but eight strains (i.e., Nec10, Ph38, Ph49, fic9, Fic72, R13, OT33 and Ap1) were pathogenic on tomato and sunflower seedlings in greenhouse conditions, while all but three strains (i.e. fic9, Fic72, and OT33) showed tumorigenicity on carrot root discs. The phylogenetic analysis and nucleotide diversity statistics suggested the existence of two novel genomospecies within A. tumefaciens species complex, which we named "G19" and "G20". Hence, we propose the strains Rew, Rnw and Rnr as the members of "G19" and the strains R13 and OT33 as the members of G20, while the phylogenetic status of the atypical strain Ap1 remains undetermined.


Journal Details

This article was published in the following journal.

Name: Phytopathology
ISSN: 0031-949X


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