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To investigate the effect of electrical pulse stimulation (EPS) on lipid accumulation and alteration of fatty acid-related enzymes in C2C12 myotubes incubated with fatty acids. Mouse C2C12 myotubes were incubated with oleic acid and palmitic acid, and differentiated C2C12 myotubes were treated with EPS, oil-red O (ORO), BODIPY staining and triglyceride (TG) content were examined. Total RNA was isolated, and real-time polymerase chain reaction analysis was performed. (1) EPS decreased TG content ( < .01). (2) EPS significantly induced the mRNA expression of FAD/CD36 ( < .05), FATP4 ( < .001), FABP1 ( < .01) and FABP5 ( < .01). (3) EPS significantly inhibited the mRNA expression of fatty acid synthase ( < .01). (4) Adipose triglyceride lipase and hormone-sensitive lipase expression were significantly elevated ( < .001), and induced the mRNA expression of CPT1 ( < .01), ACOX1 ( < .05), UCP3 ( < .05) and PPARα ( < .001) after EPS. EPS reduced lipid droplet accumulation; enhanced CD36, FATP4, FABP1 and FABP5 expression; inhibited C2C12 myotube fatty acid re-esterification; and promoted fatty acid oxidation in C2C12 myotubes.
This article was published in the following journal.
Name: Archives of physiology and biochemistry
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