Topics

Decorating the Outer Surface of Microbially Produced Protein Nanowires with Peptides.

08:00 EDT 12th July 2019 | BioPortfolio

Summary of "Decorating the Outer Surface of Microbially Produced Protein Nanowires with Peptides."

The potential applications of electrically conductive protein nanowires (e-PNs) harvested from Geobacter sulfurreducens might be greatly expanded if the outer surface of the wires could be modified to confer novel sensing capabilities or to enhance binding to other materials. We developed a simple strategy for functionalizing e-PNs with surface-exposed peptides. The G. sulfurreducens gene for the monomer that assembles into e-PNs was modified to add peptide tags at the carboxyl terminus of the monomer. Strains of G. sulfurreducens were constructed that fabricated synthetic e-PNs with a six-histidine 'His-tag' or both the His-tag and a nine-peptide 'HA-tag' exposed on the outer surface. Addition of the peptide tags did not diminish e-PN conductivity. The abundance of HA-tag in e-PNs was controlled by placing expression of the gene for the synthetic monomer with the HA-tag under transcriptional regulation. These studies suggest broad possibilities for tailoring e-PN properties for diverse applications.

Affiliation

Journal Details

This article was published in the following journal.

Name: ACS synthetic biology
ISSN: 2161-5063
Pages:

Links

DeepDyve research library

PubMed Articles [18623 Associated PubMed Articles listed on BioPortfolio]

Stirring revealed new functions of ethylenediamine and hydrazine in the morphology control of copper nanowires.

Cu nanowires, as promising candidates in many fields because of their merits, are commonly prepared by the solution phase based synthesis which is a simple and scalable method. However, precise contro...

Atomistic Mechanism of Stress-Induced Combined Slip and Diffusion in Sub-5 Nanometer Sized Ag Nanowires.

With continuous minimization of nano-devices, the dimensions of metallic materials used in nano-devices decrease to a few nanometers. Understanding the structural stability and deformation behavior of...

Localized incorporation of outer membrane components in the pathogen .

The zoonotic pathogen is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth. Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition...

Osteoblast adhesion and response mediated by terminal -SH group charge surface of SiOxCy nanowires.

Robust cell adhesion is known to be necessary to promote cell colonization of biomaterials and differentiation of progenitors. In this paper, we propose the functionalization of Silicon Oxycarbide (Si...

Role of Ligand Conformation on Nanoparticle-Protein Interactions.

Engineered biomedical nanoparticles (NP) administered via intravenous routes are prone to associate to serum proteins. The protein corona can mask the NP surface functionalization and hamper the deliv...

Clinical Trials [4315 Associated Clinical Trials listed on BioPortfolio]

Differences in Muscle Activity Patterns and Graphical Product Quality in Children With Graphomotor Impairment Copying and Tracing Activities on Horizontal or Vertical Surfaces

Drawing on a vertical surface, such as a blackboard (rather than a horizontal surface) is often used by occupational therapists as a way of developing fine motor control and visual motor i...

Syndecan-1 a Surrogate Marker for IBD

Syndecan-1 is a protein on the surface intestinal cells. previous studies proved low levels of mucosal syndecan-1 levels on the surface of intestinal cells is patients with acute and chron...

Noninvasive VNS for Neuromotor Adaptations

The study will examine how electrical stimulation of vagus nerve (i.e. nerve around the outer ear) from the skin surface during motor training influences a brain hormone (called norepineph...

Safety and Efficacy of MBI 226 for the Prevention of Central Venous Catheter-Related Bloodstream Infections

Central venous catheters (CVCs) are used in patient care for such purposes as the administration of medication, fluids, blood products and for functions such as hemodialysis and plasmapher...

Efficacy of Autologous Outer-Root-Sheath Melanocytes Transplantation in the Treatment of Vitiligo

The purpose of this study is to evaluate the hypothesis that, in patients with stable vitiligo unresponsive to conventional treatments including traditional phototherapy and/or topical ste...

Medical and Biotech [MESH] Definitions

The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

Gram-negative bacterial secretion systems which translocate effectors in a single step across the inner and outer membranes. The one-step secretion is carried out by a channel that passes from the CYTOPLASM, through the inner membrane, PERIPLASMIC SPACE, and outer membrane, to the EXTRACELLULAR SPACE. The specificity of type I secretions systems are determined by the specificity of the three subcomponents forming the channel - an ATP transporter (ATP-BINDING CASSETTE TRANSPORTERS); a membrane fusion protein (MEMBRANE FUSION PROTEINS); and an outer membrane protein (BACTERIAL OUTER MEMBRANE PROTEINS.)

A lesion on the surface of the skin or a mucous surface, produced by the sloughing of inflammatory necrotic tissue.

Materials which have structured components with at least one dimension in the range of 1 to 100 nanometers. These include NANOCOMPOSITES; NANOPARTICLES; NANOTUBES; and NANOWIRES.

A gelatinous membrane overlying the acoustic maculae of SACCULE AND UTRICLE. It contains minute crystalline particles (otoliths) of CALCIUM CARBONATE and protein on its outer surface. In response to head movement, the otoliths shift causing distortion of the vestibular hair cells which transduce nerve signals to the BRAIN for interpretation of equilibrium.

Quick Search


DeepDyve research library

Relevant Topics

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


Searches Linking to this Article