Interferometric scattering microscopy: seeing single nanoparticles and molecules via Rayleigh scattering.

08:00 EDT 17th July 2019 | BioPortfolio

Summary of "Interferometric scattering microscopy: seeing single nanoparticles and molecules via Rayleigh scattering."

Fluorescence microscopy has been the workhorse for investigating optical phenomena at the nanometer scale, but this approach confronts several fundamental limits. As a result, there have been a growing number of activities toward the development of fluorescent-free imaging methods. In this mini-review, we demonstrate that elastic scattering, the most ubiquitous and oldest optical contrast mechanism, offers excellent opportunities for sensitive detection and imaging of nanoparticles and molecules at very high spatio-temporal resolution. We present interferometric scattering (iSCAT) microscopy as the method of choice, explain its theoretical foundation, discuss its experimental nuances and elaborate on its deep connection to bright-field imaging and several other established microscopies. A showcase of numerous applications and avenues made possible by iSCAT demonstrates its rapidly growing impact on various disciplines concerned with nanoscopic phenomena.


Journal Details

This article was published in the following journal.

Name: Nano letters
ISSN: 1530-6992


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