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Interferometric scattering microscopy: seeing single nanoparticles and molecules via Rayleigh scattering.

08:00 EDT 17th July 2019 | BioPortfolio

Summary of "Interferometric scattering microscopy: seeing single nanoparticles and molecules via Rayleigh scattering."

Fluorescence microscopy has been the workhorse for investigating optical phenomena at the nanometer scale, but this approach confronts several fundamental limits. As a result, there have been a growing number of activities toward the development of fluorescent-free imaging methods. In this mini-review, we demonstrate that elastic scattering, the most ubiquitous and oldest optical contrast mechanism, offers excellent opportunities for sensitive detection and imaging of nanoparticles and molecules at very high spatio-temporal resolution. We present interferometric scattering (iSCAT) microscopy as the method of choice, explain its theoretical foundation, discuss its experimental nuances and elaborate on its deep connection to bright-field imaging and several other established microscopies. A showcase of numerous applications and avenues made possible by iSCAT demonstrates its rapidly growing impact on various disciplines concerned with nanoscopic phenomena.

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This article was published in the following journal.

Name: Nano letters
ISSN: 1530-6992
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