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Accurate and efficient segmentation of cell nuclei in fluorescence microscopy images plays a key role in many biological studies. Besides coping with image noise and other imaging artifacts, the separation of touching and partially overlapping cell nuclei is a major challenge. To address this, we introduce a globally optimal model-based approach for cell nuclei segmentation which jointly exploits shape and intensity information. Our approach is based on implicitly parameterized shape models, and we propose single-object and multi-object schemes. In the single-object case, the used shape parameterization leads to convex energies which can be directly minimized without requiring approximation. The multi-object scheme is based on multiple collaborating shapes and has the advantage that prior detection of individual cell nuclei is not needed. This scheme performs joint segmentation and cluster splitting. We describe an energy minimization scheme which converges close to global optima and exploits convex optimization such that our approach does not depend on the initialization nor suffers from local energy minima. The proposed approach is robust and computationally efficient. In contrast, previous shape-based approaches for cell segmentation either are computationally expensive, not globally optimal, or do not jointly exploit shape and intensity information. We successfully applied our approach to fluorescence microscopy images of five different cell types and performed a quantitative comparison with previous methods.
This article was published in the following journal.
Name: Medical image analysis
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A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
Microscopy in which television cameras are used to brighten magnified images that are otherwise too dark to be seen with the naked eye. It is used frequently in TELEPATHOLOGY.
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore. Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.
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