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Synthetic gene circuits enable Escherichia coli to use endogenous H2S as a signaling molecule for quorum sensing.

08:00 EDT 6th August 2019 | BioPortfolio

Summary of "Synthetic gene circuits enable Escherichia coli to use endogenous H2S as a signaling molecule for quorum sensing."

Microorganisms often use specific autoinducers other than common metabolites for quorum sensing (QS). Herein, we demonstrated that Escherichia coli produced sulfide (H2S, HS-, and S2-) with the concentrations proportionally correlated to its cell density. We then designed synthetic gene circuits that used H2S as an autoinducer for quorum sensing. A sulfide:quinone oxidoreductase converted diffusible H2S to indiffusible hydrogen polysulfide (HSnH, n≥2), and a gene regulator CstR sensed the latter to turn on gene expression. We constructed three element libraries, with which 24 different circuits could be assembled for adjustable sensitivity to cell density. The H2S-mediated gene circuits endowed E. coli cells within the same batch or microcolony with highly synchronous behaviors. Using them we successfully constructed cell factories capable of autonomous switch from growth phase to production phase. Thus, these circuits provide a new tool-kit for metabolic engineering and synthetic biology.

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This article was published in the following journal.

Name: ACS synthetic biology
ISSN: 2161-5063
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