CryoEM maps are full of potential.

08:00 EDT 7th August 2019 | BioPortfolio

Summary of "CryoEM maps are full of potential."

Electron microscopy is based on elastic scattering due to Coulomb forces between the incident electrons and the sample; thus, electron scattering is dependent on the charge distribution in the sample. Unlike atomic scattering factors for X-rays, electron scattering factors for some atoms are strongly dependent on scattering angle, and the scattering factor for ionic oxygen is negative at low scattering angle. This phenomenon can result in a significant negative contribution to Coulomb potential maps by oxygen and can result in deviations in the positions of positive map features from atomic centers. An important factor that can also complicate the interpretation of cryoEM maps is the exquisite sensitivity of macromolecules to damage from electron irradiation, especially the carboxylates of acidic amino acids. Ideally, when compared with electron density maps derived by X-ray crystallography, Coulomb potential maps can provide additional details about the electrostatic environment and charge state of atoms. Enhancements in model building, refinement and computational simulation will be required to realize the full potential of EM-derived maps to reveal deeper insight into the electronic structure and functional properties of macromolecular complexes and their interactions with binding partners, ligands, cofactors, and drugs.


Journal Details

This article was published in the following journal.

Name: Current opinion in structural biology
ISSN: 1879-033X


DeepDyve research library

PubMed Articles [10828 Associated PubMed Articles listed on BioPortfolio]

Integration of Cryo-EM Model Building Software in Scipion.

Advances in cryo-electron microscopy (cryoEM have made possible to obtain structures of large biological macromolecules at near-atomic resolution. This "resolution-revolution'' has encouraged the use ...

Synthetic antibodies against BRIL as universal fiducial marks for single-particle cryoEM structure determination of membrane proteins.

We propose the concept of universal fiducials based on a set of pre-made semi-synthetic antibodies (sABs) generated by customized phage display selections against the fusion protein BRIL, an engineer...

Haruspex: A Neural Network for the Automatic Identification of Oligonucleotides and Protein Secondary Structure in Cryo-EM Maps.

In recent years, three-dimensional density maps reconstructed from single particle images obtained by electron cryo-microscopy (Cryo-EM) have reached unprecedented resolution. However, map interpretat...

Current trends in marine algae polysaccharides: The digestive tract, microbial catabolism, and prebiotic potential.

Marine algae contain unique polysaccharides which differ from terrestrial plant polysaccharides, having a different composition, substitution, and linkage types. These are non-digestible by digestive ...

Prediction of cognitive performance in old age from spatial probability maps of white matter lesions.

The purposes of this study were to explore the association between cognitive performance and white matter lesions (WMLs), and to investigate whether it is possible to predict cognitive impairment usin...

Clinical Trials [3119 Associated Clinical Trials listed on BioPortfolio]

Combined Treatment for Tobacco and Alcohol Use in Puerto Rico

Objectives: 1. Assess MAPS and MAPS+ effects on alcohol at-risk behaviors and smoking cessation. 2. Assess MAPS and MAPS+ effects on treatment mechanisms (increased self-efficacy, ...

Reducing Tobacco Related Health Disparities

The goal of this research study is to learn the effectiveness of telephone-based counseling on helping smokers change their smoking patterns. Researchers want to learn if telephone counse...

Enhanced Linkage Maps From Family-Based Genetics Studies

To develop genetic maps for the genome-wide screening markers used by multiple investigators and to investigate map differences between sexes and different ethnic groups.

Metabolic Characteristics of Sarcoma In Vivo Using HP 13-C Magnetic Resonance Spectroscopic Imaging (MRSI)

Investigators will determine whether MRSI using hyperpolarized [1-13C]pyruvate provide useful information of tumor malignancy in MRI-indeterminate cases. Metabolite maps of hyperpolarized ...

MAPS Group Therapy Model for Bipolar Disorder

This study aims to develop the MAPS(M,Monitoring;A,Assessing;P,Preventing or Reducing Relapse;S,Smart goal setting) group therapy model for bipolar disorder patients as other adjuvant clin...

Medical and Biotech [MESH] Definitions

Opportunity to attain full health potential and no one is disadvantaged from achieving this potential because of their social position or other socially determined circumstance.

Recovery from mental disorders and/or substance abuse. The process of change in which individuals improve their MENTAL HEALTH and wellness, live a self-directed life, and work to achieve their full potential.

Solution titration in which the end point is read from the electrode-potential variations with the concentrations of potential determining ions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Graphs representing sets of measurable, non-covalent physical contacts with specific PROTEINS in living organisms or in cells.

Representations, normally to scale and on a flat medium, of a selection of material or abstract features on the surface of the earth, the heavens, or celestial bodies.

Quick Search

DeepDyve research library

Relevant Topic

Radiology is the branch of medicine that studies imaging of the body; X-ray (basic, angiography, barium swallows), ultrasound, MRI, CT and PET. These imaging techniques can be used to diagnose, but also to treat a range of conditions, by allowing visuali...

Searches Linking to this Article