Topics

Leaf-Inspired Authentically Complex Microvascular Networks for Deciphering Biological Transport Processes.

08:00 EDT 14th August 2019 | BioPortfolio

Summary of "Leaf-Inspired Authentically Complex Microvascular Networks for Deciphering Biological Transport Processes."

The vascular transport of molecules, cells and nanoconstructs is a fundamental biophysical process impacting tissue regeneration; delivery of nutrients and therapeutic agents; and the response of the immune system to external pathogens. This process is often studied in single-channel microfluidic devices lacking the complex tri-dimensional organization of vascular networks. Here, soft lithography is employed to replicate the vein system of a Hedera elix leaf on a polydimethilsiloxane (PDMS) template. The replica is then sealed and connected to an external pumping system to realize an authentically complex microvascular network. This satisfies energy minimization criteria by the Murray's law and comprises a network of channels ranging in size from capillaries ( 50 m) to large arterioles and venules ( 400 m). Micro-PIV (Micro - Particle Image Velocimetry) analysis is employed to characterize flow conditions in terms of streamlines, fluid velocity, and flow rates. To demonstrate the ability to reproduce physiologically relevant transport processes, two different applications are demonstrated: vascular deposition of tumor cells and lysis of blood clots. To this end, conditions are identified to culture cells within the microvasculature and realize a confluent endothelial monolayer. Then, the vascular deposition of circulating breast (MDA-MB 231) cancer cells is documented throughout the network under physiologically relevant flow conditions. Firm cell adhesion mostly occurs in channels with low mean blood velocity. As a second application, blood clots are formed within the chip by mixing whole blood with a thrombin solution. After demonstrating the blood clot stability, tissue plasminogen activator (tPA) and tPA-carrying nanoconstructs (tPA-DPNs) are employed as thrombolytics. In agreement with previous data, clot dissolution is equally induced by tPA and tPA-DPNs. The proposed leaf-inspired chip can be efficiently used to study a variety of vascular transport processes in complex microvascular networks, where geometry and flow conditions can be modulated and monitored throughout the experimental campaign.

Affiliation

Journal Details

This article was published in the following journal.

Name: ACS applied materials & interfaces
ISSN: 1944-8252
Pages:

Links

DeepDyve research library

PubMed Articles [10456 Associated PubMed Articles listed on BioPortfolio]

Computational methods for identifying the critical nodes in biological networks.

A biological network is complex. A group of critical nodes determines the quality and state of such a network. Increasing studies have shown that diseases and biological networks are closely and mutua...

ANCA: Alignment-based Network Construction Algorithm.

Dynamic biological networks model changes in the network topology over time. However, often the topologies of these networks are not available at specific time points. Existing algorithms for studying...

Ollivier-Ricci Curvature-Based Method to Community Detection in Complex Networks.

Identification of community structures in complex network is of crucial importance for understanding the system's function, organization, robustness and security. Here, we present a novel Ollivier-Ric...

Leaf venation networks of Bornean trees: images and hand-traced segmentations.

The data set contains images of leaf venation networks obtained from tree species in Malaysian Borneo. The data set contains 726 leaves from 295 species comprising 50 families, sampled from 8 forest p...

Leaf morphology, rather than plant water status, underlies genetic variation of rice leaf rolling under drought.

Soil drying causes leaf rolling in rice, but the relationship between leaf rolling and drought tolerance has historically confounded selection of drought tolerant genotypes. In this study on tropical ...

Clinical Trials [3130 Associated Clinical Trials listed on BioPortfolio]

Mulberry Leaf Extract and Blood Glucose Control in Diabetics

Mulberry leaf teas or extracts are used as a natural remedy for diabetes in some countries. Animal studies have shown some effect of mulberry leaf extract on blood glucose. The purpose of ...

Life Enhancing Activities for Family Caregivers (LEAF 2.0)

The goal of the proposed intervention, called LEAF (Life Enhancing Activities for Family Caregivers) is to reduce burden and increase well-being in Alzheimer's Disease caregivers through t...

The Use of Silver Leaf Dressing in the Prevention of Radiotherapy Induced Skin Reactions

The hypothesis is that silver leaf nylon dressing reduces the percentage of patients developing brisk erythema and moist desquamation while undergoing whole breast radiotherapy. The secon...

Anti-diabetic Effects of Persimmon Leaf Extract

The investigators performed a 8-week, randomized, double-blind, placebo-controlled crossover human trial to evaluate the efficacy and safety of persimmon leaf extract on blood glucose. The...

Use of Banana Leaf Dressing on Donor Site Wounds

Prospective Randomized double-blind controlled trial comparing the healing rate, infection rate, pain score, ease of care and scar formation of Banana leaf dressing versus hydrocolloid dre...

Medical and Biotech [MESH] Definitions

Complex pharmaceutical substances, preparations, or agents of organic origin, usually obtained by biological methods or assay. They are used especially in diagnosis and treatment of disease (as vaccines or pollen extracts). Biological products are differentiated from BIOLOGICAL FACTORS in that the latter are compounds with biological or physiological activity made by living organisms. (From Webster's 3d ed)

Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.

A computer architecture, implementable in either hardware or software, modeled after biological neural networks. Like the biological system in which the processing capability is a result of the interconnection strengths between arrays of nonlinear processing nodes, computerized neural networks, often called perceptrons or multilayer connectionist models, consist of neuron-like units. A homogeneous group of units makes up a layer. These networks are good at pattern recognition. They are adaptive, performing tasks by example, and thus are better for decision-making than are linear learning machines or cluster analysis. They do not require explicit programming.

The study of ancient inscriptions and modes of writing. It includes the deciphering of manuscripts and other forms to determine their date, provenance, etc. (Webster's 1st ed)

Techniques for standardizing and expediting taxonomic identification or classification of organisms that are based on deciphering the sequence of one or a few regions of DNA known as the "DNA barcode".

Quick Search


DeepDyve research library

Relevant Topics

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Vascular
Vascular relates to blood vessels (Oxford Medical Dictionary) and can be used to describe the supply of blood, a disease affecting the blood vessels or molecules associated with these structures. For example, <!--LGfEGNT2Lhm-->atherosclerosis ...

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Article