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Pharmacokinetics, bioavailability and urinary excretion of scopolin and its metabolite scopoletin in Sprague-Dawley rats by LC-MS/MS.

08:00 EDT 14th August 2019 | BioPortfolio

Summary of "Pharmacokinetics, bioavailability and urinary excretion of scopolin and its metabolite scopoletin in Sprague-Dawley rats by LC-MS/MS."

The aim of this study is to investigate the pharmacokinetics, bioavailability and urinary excretion of scopolin and its metabolite scopoletin in rat. A LC-MS/MS method for simultaneous determination of scopolin and scopoletin in rat biomatrices was developed and validated over the rage of 5.0-2000 ng/mL. Chromatographic separation was carried out on a Hypersil GOLD C18 column with acetonitrile and 0.1% formic acid in water as mobile phase with gradient elution. Detection was performed in the positive ionization and selected reaction monitoring mode. The intra- and inter- batch precision and accuracy, extraction recovery and matrix effect, stability of scopolin and scopoletin were well within the acceptable limits of variation. There was no gender-related difference in pharmacokinetic profiles of scopolin. There were significant differences in AUC, T and Cl/F of scopoletin between the male and female rats (p < 0.05). The bioavailability of scopolin was exceptionally low. The maximal excretion rates were 7.61 μg/h and 7.15 μg/h for scopolin and 31.68 μg/h and 25.58 μg/h for scopoletin in male and female rats, respectively. The LC-MS/MS method was successfully applied to the pharmacokinetic, bioavailability and urinary excretion studies of scopolin and its metabolite scopoletin following single administration of scopolin to rats.

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This article was published in the following journal.

Name: Biomedical chromatography : BMC
ISSN: 1099-0801
Pages: e4678

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