Identification and Functional Analysis of a Delta Class Glutathione S-transferase Gene Associated with Insecticide Detoxification in .

08:00 EDT 14th August 2019 | BioPortfolio

Summary of "Identification and Functional Analysis of a Delta Class Glutathione S-transferase Gene Associated with Insecticide Detoxification in ."

A delta class GST gene () is identified from for the first time. Developmental expression analysis showed expression of is significantly higher in the fourth instar larval stage and in the adult stage. Tissue-specific expression analysis found that was expressed predominantly in the midgut and Malpighian tubules in the fourth-instar larvae and in the abdomen of adults. Expression of was significantly upregulated following exposure to chlorpyrifos and clothianidin. inhibition and metabolic assays indicated that recombinant BoGSTd2 could not directly metabolize chlorpyrifos and clothianidin. Nevertheless, disk diffusion assays indicated that BoGSTd2 plays an important role in protection against oxidative stress. RNAi assays showed that participates in the elimination of ROS induced by chlorpyrifos and clothianidin. These results strongly suggest that plays an important role in chlorpyrifos and clothianidin detoxification in by protecting tissues from oxidative stress induced by these insecticides.


Journal Details

This article was published in the following journal.

Name: Journal of agricultural and food chemistry
ISSN: 1520-5118


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Medical and Biotech [MESH] Definitions

A glutathione transferase that catalyzes the conjugation of electrophilic substrates to GLUTATHIONE. This enzyme has been shown to provide cellular protection against redox-mediated damage by FREE RADICALS.

A transferase that catalyzes the addition of aliphatic, aromatic, or heterocyclic FREE RADICALS as well as EPOXIDES and arene oxides to GLUTATHIONE. Addition takes place at the SULFUR. It also catalyzes the reduction of polyol nitrate by glutathione to polyol and nitrite.

MOLECULAR BIOLOGY techniques used in the diagnosis of disease. Included are such techniques as IN SITU HYBRIDIZATION of chromosomes for CYTOGENETIC ANALYSIS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS of gene expression patterns in disease states; identification of pathogenic organisms by analysis of species specific DNA sequences; and detection of mutations with POLYMERASE CHAIN REACTION.

Conjugation of exogenous substances with various hydrophilic substituents to form water soluble products that are excretable in URINE. Phase II modifications include GLUTATHIONE; ACYLATION; and AMINATION. Phase II enzymes include GLUTATHIONE TRANSFERASE and GLUCURONOSYLTRANSFERASE. In a sense these reactions detoxify phase I reaction products.

Catalyzes the oxidation of GLUTATHIONE to GLUTATHIONE DISULFIDE in the presence of NADP+. Deficiency in the enzyme is associated with HEMOLYTIC ANEMIA. Formerly listed as EC

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