Topics

Heterologous Expression of Pteris vittata Phosphate Transporter PvPht1;3 Enhances Arsenic Translocation to and Accumulation in Tobacco Shoots.

08:00 EDT 14th August 2019 | BioPortfolio

Summary of "Heterologous Expression of Pteris vittata Phosphate Transporter PvPht1;3 Enhances Arsenic Translocation to and Accumulation in Tobacco Shoots."

Arsenic-hyperaccumulator Pteris vittata is efficient in As accumulation and has been used in phytoremediation of As-contaminated soils. Arsenate (AsV) is the predominant As species in soils and is taken up by plants via phosphate transporters (Pht). In this work, we cloned the PvPht1;3 full length coding sequence from P. vittata and investigated its role in As accumulation by yeast and plants. PvPht1;3 complemented a yeast P uptake mutant and showed a stronger affinity and transport capacity to AsV than PvPht1;2. In transgenic tobacco, PvPht1;3 enhanced AsV absorption and translocation, increasing As accumulation in the shoots under both hydroponic and soil cultivation. Based on the expression patterns via qRT-PCR, PvPht1;3 was strongly induced by P deficiency, but not As exposure. To further understand its expression pattern, transgenic Arabidopsis and soybean expressing the GUS reporter gene, driven by PvPht1;3 promoter, were produced. The GUS staining showed that the reporter gene was mainly expressed in the stele cells, indicating that PvPht1;3 was expressed in stele cells and was likely involved in P/As translocation . Taken together, the data suggested that PvPht1;3 was a high-affinity AsV transporter and was probably responsible for efficient As translocation in P. vittata. Our results suggest that expressing PvPht1;3 enhances As translocation and accumulation in plants, thereby improving phytoremediation of As-contaminated soils.

Affiliation

Journal Details

This article was published in the following journal.

Name: Environmental science & technology
ISSN: 1520-5851
Pages:

Links

DeepDyve research library

PubMed Articles [13532 Associated PubMed Articles listed on BioPortfolio]

Interactive effects of As, Cd and Zn on their uptake and oxidative stress in As-hyperaccumulator Pteris vittata.

The effects of arsenic (As), cadmium (Cd) and zinc (Zn) on each other's uptake and oxidative stress in As-hyperaccumulator Pteris vittata were investigated. P. vittata plants were exposed to 50 μM ...

Anaerobic digestion to reduce biomass and remove arsenic from As-hyperaccumulator Pteris vittata.

The lack of efficient methods to treat As-rich biomass is a drawback for phytoremediation technology. In this study, we applied anaerobic digestion to reduce biomass and remove As from As-rich Pteris ...

Potential use of the Pteris vittata arsenic hyperaccumulation-regulation network for phytoremediation.

Arsenic accumulation in soil is a global problem typically addressed using phytoremediation methods. Pteris vittata, a model arsenic hyperaccumulator, has great potential as a genetically engineered p...

Arsenic removal and biomass reduction of As-hyperaccumulator Pteris vittata: Coupling ethanol extraction with anaerobic digestion.

Improper disposal of arsenic-rich biomass and the lack of efficient methods to treat it may cause contamination in the environment. We developed an efficient method for arsenic (As) removal and biomas...

Pteris melanocaulon Fée is an As hyperaccumulator.

Pteris melanocaulon is noted to thrive very well in selected Cu-Au mines in the Philippines such as the Carmen Cu mine in Cebu and the Manila Mining Cu-Au mine in Surigao. Soil and plant samples were ...

Clinical Trials [3042 Associated Clinical Trials listed on BioPortfolio]

Intestinal Phosphate Transporter Expression in CKD Patients

The aim of the present study is to study the expression of the various phosphate transporters in patients with normal or moderately impaired renal function or in patients on dialysis. In p...

Ontogenesis of the P-Glycoprotein in Human Lymphocytes Influence of HIV and Antiretroviral Therapeutics

The P-glycoprotein (P-gp) is a membranous transporter that modulates the intracellular concentrations of many drugs and plays thus a major role in the efficacy of the therapeutics that act...

The Impact of a Low or High Phosphate Diet on Phosphate and Calcium Excretion in Healthy People

Phosphate is present in many of the foods that the investigators eat and is required by many cellular processes. The kidneys are the only organ that excrete the extra phosphate from the di...

A Window of Opportunity Study of Methionine Deprivation in Triple Negative Breast Cancer

Given preliminary data demonstrating that methionine deprivation enhances cell surface expression of TRAIL receptor-2, the objective of this clinical trial is to confirm that methionine re...

Expression of BCRP in Icteric Patients

The expression of transporters involved in bile acid homeostasis is differentially regulated during obstructive cholestasis. Bile acids are also substrates of the drug efflux transporter b...

Medical and Biotech [MESH] Definitions

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

A non-electrogenic sodium-dependent phosphate transporter. It is found primarily in apical membranes of PROXIMAL RENAL TUBULES.

A sodium-dependent phosphate transporter present primarily at apical sites of EPITHELIAL CELLS in the SMALL INTESTINE.

An electrogenic sodium-dependent phosphate transporter. It is present primarily in BRUSH BORDER membranes of PROXIMAL RENAL TUBULES.

The immunological phenomenon by which exposure to some organisms or vaccines can profoundly alter the host's response to subsequent exposure to unrelated (heterologous) organisms or vaccines.

Quick Search


DeepDyve research library

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Genetic Engineering
Genetic engineering is the process of manually adding new DNA to an organism. The goal is to add one or more new traits that are not already found in that organism. Examples of genetically engineered (transgenic) organisms currently on the market include...

Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...


Searches Linking to this Article