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Methods based on the photothermal effect (a common phenomenon in nature) have been widely applied in different fields; however, their application in bioanalysis has lagged behind. Herein, we designed a near-infrared (NIR) photothermal immunoassay for the qualitative or quantitative detection of prostate-specific antigen (PSA) using titanium carbide (Ti3C2) MXene quantum dot (QD)-encapsulated liposomes with high photothermal efficiency. This system involves a sandwich-type immunoreaction and photothermal measurements. Ti3C2 MXene QDs were utilized as innovative photothermal signal beacons and were encapsulated in liposomes for the labeling of the secondary antibody. The assay was carried out by coupling a low-cost microplate with a homemade 3D printed device. Under NIR-laser irradiation, the Ti3C2 MXene QDs converted the light energy into heat, and a shift in temperature corresponding with the analyte concentration was obtained on a handheld thermometer. Under optimal conditions, the Ti3C2 MXene QD-based photothermal immunoassay exhibited a dynamic linear range from 1.0 ng mL-1 to 50 ng mL-1 with a limit of detection of 0.4 ng mL-1 for PSA detection. Also, we constructed portable equipment using a portable near-infrared imaging camera to collect visual thermal data for the semi-quantitative analysis of the target PSA within 3 min. The specificity, reproducibility and accuracy of the photothermal immunoassay were acceptable. Importantly, our strategy opens new opportunities for protein point-of-care (POC) testing and biosecurity diagnostics.
This article was published in the following journal.
Photothermal effects (PTEs) have been greatly concerned with the fast development of new photothermal nanomaterials. Herein we propose a photothermal immunoassay (PTIA) by taking mycotoxins (AFB1) as ...
Liposomes are one of the most important and extensively studied drug delivery system due to their ability to encapsulate different kinds of drugs. Exploiting the advantages of H Nuclear Magnetic Reson...
Liposomal photoelectrochemical (PEC) bioanalysis holds enormous potential for future sensitive PEC bioanalysis. With tyrosinase (Tyr) and TiO as representative enzyme and electrode, respectively, this...
We conducted photo-activated delivery of drugs based on the fusion of liposomes with endocytic membranes, thus allowing the direct release of encapsulated drugs inside the cytoplasm. As described in o...
Cinnamaldehyde is an active component of some plant essential oils, which has broad antibacterial activity. However, the strong volatility and instability of cinnamaldehyde limits its application. Cin...
The objetive of the study is to evaluate the effect of personalized quantum sonotherapy on the level of anxiety and pain in outpatients schedule for orthopedic surgery under regional anest...
The purpose of this study is: 1. To investigate the feasibility of using photothermal camera and infrared (IR) thermometer to grade the severity of oral mucositis in patients with ...
Food fortification has shown to be efficacious to alleviate the burden of micronutrient deficiencies. Ensuring the bioavailability of iron and maintaining the sensory quality and stability...
The purpose of the study is to evaluate the safety and efficacy of the composite Nail - the Quantum interlocking intramedullary nailing system in the reduction of humeral fractures.
A phase II,randomized,double-blind,multi-doses,positive drug parallel controlled,multi-center clinical trial to evaluate initially the efficacy and safety of alprostadil liposomes for inje...
Nanometer sized fragments (the dots) of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They are brighter and more persistent than organic chemical INDICATORS. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY.
The use of photothermal effects of LASERS to coagulate, incise, vaporize, resect, dissect, or resurface tissue.
A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
Single membrane vesicles, generally made of PHOSPHOLIPIDS.
A synthetic phospholipid used in liposomes and lipid bilayers for the study of biological membranes.
Immunoassay - ELISA
Immunoassays are quick and accurate tests to detect specific molecules. Immunoassays rely on an antibody to bind to the specific structure of a molecule. Antibodies are proteins generated by animals in response to the invasion of a foreign molecule (anti...
A diagnostic test is any kind of medical test performed to aid in the diagnosis or detection of disease. For example: to diagnose diseases to measure the progress or recovery from disease to confirm that a person is free from disease Clin...
Prostate cancer (cancer de prostata) Prostate cancer (cancer de prostata) is a form of cancer that develops in the prostate, a gland in the male reproductive system. Most prostate cancers are slow growing; however, there are cases of aggressive prostat...