Molecular and anatomical organization of the dorsal raphe nucleus.

08:00 EDT 14th August 2019 | BioPortfolio

Summary of "Molecular and anatomical organization of the dorsal raphe nucleus."

The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection the DRN and its diverse functions.


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This article was published in the following journal.

Name: eLife
ISSN: 2050-084X


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Medical and Biotech [MESH] Definitions

The dorsal region of the raphe nucleus which is a source of the SEROTONERGIC NEURONS innervating the TELENCEPHALON; DIENCEPHALON; MESENCEPHALON; and RHOMBENCEPHALON.

A nucleus in the medullary Raphe nuclei located rostral to the NUCLEUS RAPHE OBSCURUS and caudal to the NUCLEUS RAPHE MAGNUS.

A nucleus in the medullary Raphe nuclei located caudal to the NUCLEUS RAPHE PALLIDUS.

A nucleus located directly rostral to the NUCLEUS RAPHE OBSCURUS in the pontine part of the Raphe nuclei. Another pontine nucleus located directly rostral to the magnus nucleus is the nucleus raphe pontis.

A nucleus located at the midline of the ventral MIDBRAIN TEGMENTUM with primarily GABAergic projections to the median and dorsal raphe nuclei and the MIDBRAIN CENTRAL GRAY.

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