Track topics on Twitter Track topics that are important to you
The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection the DRN and its diverse functions.
This article was published in the following journal.
In this issue of Neuron, Sengupta and Holmes (2019) characterize a distinct serotonergic circuit from the dorsal raphe nucleus to the basal amygdala that facilitates fear conditioning and memory.
In a previous study, we showed that exposure of rats to a one-week environmental enrichment (EE) protocol decreases elevated T-maze (ETM) avoidance responses, an anxiolytic-like effect, without alteri...
Loss of the mother-infant relationship during early childhood affects infant development and is known to increase the infant's vulnerability to neuropsychiatric disorders throughout life. Serotonin de...
Many see the periaqueductal grey (PAG) as a region responsible for the downstream control of defensive reactions. Here we provide a detailed review of anatomical and functional data on the different p...
Serotonergic agents have been widely used for treatment of psychiatric disorders, but the therapeutic effects are insufficient and these drugs often induce severe side effects. We need to specify the ...
This is a multi-site, prospective, open-label, randomized, 2 period cross over study comparing the subjects' current 32G pen needle (four groups of pen needles) to the BD Nucleus pen needl...
Drooping of nucleus is a common complication during cataract extraction by phacoemulsification. The investigators describe a new technique to retrieve the dropped nucleus by using an instr...
Delinieation of the anatomical location, cellular origin and molecular basis of gut-derived glucagon secretion
The purpose of this study is to evaluate whether the Iowa/Nucleus 10/10 mm in one ear, in conjunction with a Nucleus Freedom implant in the other ear can provide useful binaural hearing in...
This study objectives are to compare the efficacy and safety between surgical excision and manual rupture for dorsal carpal ganglion.The participants with dorsal carpal ganglion will be ra...
The dorsal region of the raphe nucleus which is a source of the SEROTONERGIC NEURONS innervating the TELENCEPHALON; DIENCEPHALON; MESENCEPHALON; and RHOMBENCEPHALON.
A nucleus in the medullary Raphe nuclei located rostral to the NUCLEUS RAPHE OBSCURUS and caudal to the NUCLEUS RAPHE MAGNUS.
A nucleus in the medullary Raphe nuclei located caudal to the NUCLEUS RAPHE PALLIDUS.
A nucleus located directly rostral to the NUCLEUS RAPHE OBSCURUS in the pontine part of the Raphe nuclei. Another pontine nucleus located directly rostral to the magnus nucleus is the nucleus raphe pontis.
A nucleus located at the midline of the ventral MIDBRAIN TEGMENTUM with primarily GABAergic projections to the median and dorsal raphe nuclei and the MIDBRAIN CENTRAL GRAY.
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...