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Fluorescent lipid probes are commonly used to label membranes of cells and model membranes like giant vesicles, liposomes, and supported lipid bilayers (SLB). Here we show that excitation of fluorescent lipid probes with BODIPY-like conjugates results in a significant acceleration of the rupture and SLB formation process for unsaturated phospholipid vesicles on SiO surfaces. The resulting SLBs also have smaller measured masses, which is indicative of a reduction in membrane thickness and/or membrane density. Excitation of fluorescent probes with NBD and Texas Red conjugates does not accelerate the SLB formation process. In the absence fluorescent probes or light, inclusion of oxidized phospholipids also accelerates SLB formation. The excitation-induced acceleration caused by BODIPY-like probes is eliminated when the probes are present with saturated phospholipids not susceptible to oxidation, and it is attenuated when a lipophilic antioxidant (α-tocopherol) is present. These results suggest that BODIPY-phospholipid conjugates are photosensitizers, and their excitation causes oxidation of lipid membranes which significantly alters membrane properties.
This article was published in the following journal.
Name: Langmuir : the ACS journal of surfaces and colloids
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