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Connexin 43 Functions as a Positive Regulator of Stem Cell Differentiation into Definitive Endoderm and Pancreatic Progenitors.

08:00 EDT 24th July 2019 | BioPortfolio

Summary of "Connexin 43 Functions as a Positive Regulator of Stem Cell Differentiation into Definitive Endoderm and Pancreatic Progenitors."

Efficient stem cell differentiation into pancreatic islet cells is of critical importance for the development of cell replacement therapies for diabetes. Here, we identify the expression pattern of connexin 43 (Cx43), a gap junction (GJ) channel protein, in human embryonic stem cell (hESC)-derived definitive endoderm (DE) and primitive gut tube cells, representing early lineages for posterior foregut (PF), pancreatic progenitors (PP), pancreatic endocrine progenitors (PE), and islet cells. As the function of GJ channels is dependent on their gating status, we tested the impact of supplementing hESC-derived PP cell cultures with AAP10, a peptide that promotes Cx43 GJ channel opening. We found that this treatment promotes the expression of DE markers FoxA2 and Sox17, leads to a more efficient derivation of DE, and improves the yield of PF, PP, and PE cells. These results demonstrate a functional involvement of GJ channels in the differentiation of embryonic stem cells into pancreatic cell lineages.

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This article was published in the following journal.

Name: iScience
ISSN: 2589-0042
Pages: 450-460

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Medical and Biotech [MESH] Definitions

A homeodomain protein and transcription regulator that functions in BLASTOCYST INNER CELL MASS and EMBRYONIC STEM CELL proliferation and CELL SELF RENEWAL. It confers pluripotency on embryonic stem cells and prevents their differentiation towards extraembryonic ENDODERM and trophectoderm (TROPHOBLAST) CELL LINEAGES.

A gap junction beta subunit that forms heteromeric hemichannels when paired with alpha subunits such as connexin-40 or CONNEXIN 43. Mutations in the connexin 30 gene (GJ6B) are associated with CLOUSTON'S SYNDROME and some hereditary forms of deafness.

A 43-kDa peptide which is a member of the connexin family of gap junction proteins. Connexin 43 is a product of a gene in the alpha class of connexin genes (the alpha-1 gene). It was first isolated from mammalian heart, but is widespread in the body including the brain.

A sialomucin protein that functions as a cell adhesion molecule. It is a negative regulator of certain types of HEMATOPOIETIC STEM CELLS.

A sialomucin protein that functions as a cell adhesion molecule. It is a negative regulator of certain types of HEMATOPOIETIC STEM CELLS.

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