Development of dual priming oligonucleotide-polymerase chain reaction (DPO-PCR) for detection of wheat component in foods.

08:00 EDT 31st July 2019 | BioPortfolio

Summary of "Development of dual priming oligonucleotide-polymerase chain reaction (DPO-PCR) for detection of wheat component in foods."

This research is aimed to establish a DPO-PCR method for rapid detection of wheat component in foods. A pair of highly specific DPO primers was designed using the wheat housekeeping gene GAG56D as the target gene. By optimizing the PCR reaction system, the DPO-PCR detection method for wheat component in food was established, and the specificity and sensitivity of the method were determined. The established DPO-PCR method was highly specific, and there was no cross-reactivity to 22 control samples. The sensitivity was high, and the minimum detection limit was 1 ng/uL. Tests on commercially available product samples showed that the method can effectively detect wheat component of foods. The DPO-PCR method established in this study is simple and accurate, and provides an effective detection method for wheat component in foods.


Journal Details

This article was published in the following journal.

Name: Cellular and molecular biology (Noisy-le-Grand, France)
ISSN: 1165-158X
Pages: 81-84


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