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Structural insights into the mechanism of human soluble guanylate cyclase.

08:00 EDT 12th September 2019 | BioPortfolio

Summary of "Structural insights into the mechanism of human soluble guanylate cyclase."

Soluble guanylate cyclase (sGC) is the primary nitric oxide (NO) sensor. It plays a central role in NO signalling and is implicated in many essential physiological processes and disease conditions. The binding of NO leads to a significant boost in sGC enzymatic activity. However, the mechanism of NO activation remains incompletely understood. Here we report the cryo-electron microscopy structures of the human sGC α1β1 heterodimer in different functional states. These structures revealed that the transducer module bridges the NO sensor module and the catalytic module. NO binding to the β1 heme-nitric oxide and oxygen binding (H-NOX) domain triggers the structural rearrangement of the sensor module and the bending-straightening conformational switch of the transducer module. The resulting movement of the N termini of the catalytic domains drives the structural changes within the catalytic module, which in turn boost sGC enzymatic activity.

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This article was published in the following journal.

Name: Nature
ISSN: 1476-4687
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Medical and Biotech [MESH] Definitions

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